TABLE 2.

Plasmids used in this study

PlasmidConstructionSource or reference
pCR2.1-TOPOCloning vectorInvitrogen (NV Leek, The Netherlands)
pCMB17apxalcA(p)::GFP, for N-terminal fusion of GFP to proteins of interest; contains N. crassa pyr45
pDM8GFP replaced mRFP1 in pCMB17apx34
pSCIpry4 with SfiI sites16
pNT33N-terminal half of teaA cDNA in pGADT731
pNT34N-terminal half of teaA cDNA in pGABKT731
pNT35C-terminal half of teaA cDNA in pGADT731
pSH19C-terminal half of teaA cDNA in pGABKT731
pNT60.7-kb teaA fragment in pDBM831
pNT281.5-kb teaA(p) fragment in pNT631
pNT60.7-kb teaA fragument from pNT1 inpDM831
pNT91.2-kb sepA fragment in pCMB17apx31
pDV7GFP replaced N-terminal half of YFP in pCMB17apx31
pDV8GFP replaced C-terminal half of YFP in pCMB17apx31
pYH010.7-kb teaA fragment from pNT1 in pDV731
pYH031.2-kb sepA fragment from pNT9 in pDV731
pYH061.2-kb teaC fragment in pCMB17apxThis study
pYH081.2-kb teaC fragment from pYH06 in pDV8This study
pYH14teaC deletion construct: flanking regions from pYH33 and pYH34 ligated with pry4 from pCS1This study
pYH16N-terminal half of teaC cDNA in pGADT7This study
pYH17N-terminal half of teaC cDNA in pGBK T7This study
pYH18C-terminal half of teaC cDNA in pGADT7This study
pYH19C-terminal half of teaC cDNA in pGBKT7This study
pYH241.2-kb teaC fragment in pDM8This study
pYH301.5-kb teaC(p) fragment in pYH24This study
pYH321.0-kb 5′-flanking region of teaC with SfiI site in pCR2.1-TOPOThis study
pYH331.0-kb 3′-flanking region of teaC with SfiI site in pCR2.1-TOPOThis study
pYH341.2-kb teaC fragment in pCR2.1-TOPOThis study