Regulation of the prespore cell fate in G1- and G2-phase cellsa

Phase of Ax4[cotB/GFP] cells% GFP-negative/GFP-positive slug cells% GFP-negative/ GFP-positive cells from slug anteriors% GFP-negative/ GFP-positive cells from slug anteriors after 12 h on agar
  • a To generate “G1” cells, spores were allowed to germinate and recover in growth medium for 16 h and were then washed and plated on agar. Slugs, or dissected slug anteriors, were disaggregated, and GFP-negative and -positive cell results were determined by flow cytometry. The results from a single determination for a representative experiment are shown.