TABLE 3.

mtDNA synthesis during germination and developmenta

ExptCell category and treatment prior to harvesting (treatment duration)mtDNA synthesis (% of mtDNA)
1Vegetative cells grown in medium + BrdU for one cell doubling100
2Spores germinated and recovered in medium + BrdU (16 h)36 ± 9.8
3Spores germinated and recovered in medium + BrdU (16 h) and developed − BrdU (30 h)18 ± 10
4Vegetative cells grown in medium − BrdU (16 h) and developed + BrdU (30 h)70 ± 8.5
5Spores germinated and recovered in medium − BrdU (16 h) and developed + BrdU (30 h)26 ± 9.5
6Spores germinated and recovered in medium − BrdU (16 h) and developed + BrdU (30 h); spores only were harvested34 ± 7.0
7Vegetative cells grown in medium − BrdU (16 h) and developed − BrdU (0-6 h), then + BrdU (6-30 h)23 ± 6.0
8Spores germinated and recovered in medium − BrdU (16 h) and developed − BrdU (0-6 h), then + BrdU (6-30 h)13 ± 7.0
  • a The mtDNA synthesis of developing cells was quantified from BrdU incorporation measured in experiments as described for Fig. 4. The BrdU incorporation observed in each band is shown relative to that observed during one cell cycle of G2 cells growing in liquid medium and normalized to the total amount of mtDNA in that band. Except for the experiment whose results are shown in row 6, all cells were harvested for processing.