TABLE 2.

Comparison of mass spectrometric results from protein purifications of S. cerevisiae penta-snRNP, S. pombe Cdc5-TAP, and S. pombe Prp1-TAP

S. pombeS. cerevisiaeHuman1a2a3a
snRNP core proteins
    Smb1pSmb1pSmB/B′XXX
    Smd1pSmd1pSmD1XXX
    Cwf9pSmd2pSmD2XXX
    Smd3pSmd3pSmD3XXX
    Sme1Sme1pSmE1XX
    Smf1pSmf1pSmF1XXX
    Smg1pSmG1pSmG1XXX
    Lsm2pLsm2pLSM2X
    Lsm3pLsm3pLSM3X
    Lsm4pLsm4pLSM4X
    Lsm5pLsm5pLSM5X
    Lsm6pLsm6pLSM6X
    Lsm7pLsm7pLSM7X
    Lsm8pLsm8pLSM8X
U1 snRNP proteins
    U1-70Snp1pU1-70X
    U1-AMud1pU1-AX
    U1-CYhc1pU1-CX
    ——bPrp42p——X
    Prp40pPrp40p——X
    Prp39pPrp39p——X
    SPBC23E6.01cNam8p——X
    Luc7pLuc7p——X
    ——Snu56p——X
    SPBC24E9.10Snu71p——X
U2 snRNP proteins
    U2ApLea1pU2A′XXX
    O13649Msl1pU2-B′XXX
    Sap61pPrp9pSF3a60XXX
    Sap62pPrp11pSf3a66XXX
    Sap114pPrp21pSf3a120XXX
    Sap49pHsh49pSF3b53XX
    Sap145pCus1pSF3b150XXX
    Prp12pRse1pSF3b130XXX
    Prp10pHsh155pSF3b160XXX
    Uap2pCus2pTat-SF1X
    SPBC29A3.07c——p14X
    O942901st3p/Snu17p——XX
U5 snRNP proteins
    Cwf6pPrp8pU5-220XXX
    Brr2pBrr2pU5-200XXX
    Cwf10pSnu114pU5-116XXX
    Prp28pPrp28pU5-100
    Cwf17p——U5-40XX
Tri-snRNP proteins
    Prp1pPrp6pU5-102XX
    Q09856Prp3pHPR3XX
    Q9UTC7Prp4pHPR4XX
    Snu66pSnu66pSART1XX
    Prp31pPrp31pPRP31XX
    SPAC607.03cSnu13pSNU13X
    ——Snu23p——X
    ——Prp38p——XX
    ——Spp381p——X
    Q9USR2Sad1pQ96RK9X
    DimlpDiblpU5-15XX
Ntc proteins
    Cdc5pCef1pCDC5LXX-
    Prp5p/Cwf1pPrp46pPRL1XX
    Cwf2p/Prp3pCwc2pRNPS1XX
    Cwf3pSyf1pSYF1XX
    Cwf4pSyf3pCRN1XX
    Cwf7pSnt309pSPF27XX
    Cwf8pPrp19pPRP19XX
    Cwf12pIsy1pISY1XX
    Cwf13pPrp45pSK1PXX
    ——Ntc20p——X
    O59733Syf2pGCIP-IPXX
Second-step factors
Q9Y7Y2Slu7pSLU7-X-
    Cwf5pSlt11pECM2XX
    Prp17pPrp17pPRP17X
    Prp22pPrp22pPRP22X
Other factors
    Cwf11p——O60306X
    Cwf15pCwc15pHSPC148X
    Cwf21p——SRm300X
    Cwf22pCwc22pM1F4GX
    Cwf24pCwc24pO15541X
    Cwf27pCwc27pPPIL1X
    O43031Spp2pQ9BQA8X
    Cwf14pCwc14pG10X
    Cwf16pCwc16pQ9BW85X
    Cwf18p——MGC23918X
    Cwf19p————X
    Cwf20p————X
    SPCC1281.02c——SPF30X
    Ded1p (DEAH box)Ded1pAbstraktX
  • a 1, S. cerevisiae penta-snRNP (34); 2, S. pombe Cdc5p complex (23); 3, S. pombe Prp1p-TAP purification X, protein present; −, protein not present.

  • b ——, no identified homolog.

  • c SwissProt or Entrez accession no.