TABLE 1.

Oligonucleotides used in this study

PrimerSequenceUse
C2-DFTGA TGC AAT CCC TGA TCA AAG CAA GTA ATT TCA AGT TAC AAT CAA TGG AAT TTA GAT GTC GTT TTC CCA GTC ACG ACG TTCLN3 disruption primer forward
C2-DRTCT TCG ATC TTT TCA ATG TAT CAT GAT TTT TGA GTG TGT AAC CAG CAA TGG TTG ATG TGC TGT GGA ATT GTG AGC GGA TACLN3 disruption primer reverse
METp-CLN2-FGAA GGA TCC ATG TTT CCT AAT TCA CCT GAForward primer to amplify 5′ CaCLN3 fragment inserted into pCaDIS
METP-CLN2-RGAA CTG CAG CAA AGA CTA GTC AAT CCC AAReverse primer to amplify 5′ CaCLN3 fragment inserted into pCaDIS
C2probeFCAG CAG TAA CTT TGA GGT TGForward primer to generate CaCLN3 probe
C2probeRGTC TTG GGT GAT AAT GGT GTReverse primer to generate CaCLN3 probe
RC8contTAT GCG ATT GTG GCT CAT AGT AAC GForward primer to confirm that CaCLN3 was under control of MET3
C2-ERAAG TGT ACC ATA ATT AGC ACT CReverse primer to confirm that CaCLN3 was under control of MET3
Hgc1probeFTAG TCA GCT TCC TGC ACC TCForward primer to generate HGC1 probe
Hgc1probeRGTA CCA CTA CCA CCA TTA CCReverse primer to generate HGC1 probe