TABLE 3.

Changes in expression of metabolic genes in an ace2 deletion strain under yeast- and hypha-inducing conditions

Systematic name and metabolic categoryaStandard namebDescriptionChange in expression (fold)c
YeastdHyphae
Glycolysis and reserve carbohydrates
    orf19.5348 TPS3 Regulatory subunit of trehalose-phosphate synthase−3.0−1.9
    orf19.7021 GPH1 Glycogen phosphorylase−2.4
    orf19.3278 GSY1 Glycogen synthase−4.8‡−1.8
    orf19.3325 GLG2 Self-glucosylating initiator of glycogen synthesis−3.2−1.5
    orf19.2020 HXT6 Glucose transporter−2.4−2.0
    orf19.542 HXK2 Hexokinase II−2.3−1.5
    orf19.3888 PGI1 Glucose-6-phosphate isomerase−1.8−1.6
    orf19.3967 PFK1 Phosphofructokinase (alpha subunit)−1.7
    orf19.6540 PFK2 Phosphofructokinase (beta subunit)−2.1
    orf19.4753 PFK26 6-Phosphofructose-2-kinase−1.5
    orf19.6814 TDH3 Glyceraldehyde-3-phosphate dehydrogenase−1.7‡−1.7
    orf19.3651 PGK1 3-Phosphoglycerate kinase−1.9‡−1.7
    orf19.4618 FBA1 Fructose-bisphosphate aldolase−2.0−1.5
    orf19.903 GPM1 Phosphoglycerate mutase−1.6−1.6
    orf19.395 ENO1 Enolase I−1.8‡−1.6
    orf19.5113 ADH2 Alcohol dehydrogenase−2.0−1.9
    orf19.3997 ADH1 Alcohol dehydrogenase−1.8
Gluconeogenesis
    orf19.6178 FBP1 Fructose-1,6-bisphosphatase+1.8
    orf19.7514 PCK1 Phosphoenolpyruvate carboxykinase+3.7
TCA cycle
    orf19.3097 PDA1 Pyruvate dehydrogenase (alpha subunit)+1.9
    orf19.4393 CIT1 Citrate synthase+2.5‡+2.3
    orf19.6385 ACO1 Aconitase+1.5‡+4.3
    orf19.4826 IDH1 Isocitrate dehydrogenase subunit+1.9
    orf19.5791 IDH2 Isocitrate dehydrogenase subunit+2.75
    orf19.6165 KGD1 Alpha-ketoglutarate dehydrogenase+1.5‡+1.8
    orf19.3358 LSC1 Succinyl-CoA ligase (alpha subunit)+1.5
    orf19.4022 SDH4 Succinate dehydrogenase+1.6‡
    orf19.543 FUM1Fumarate hydratase+1.6+1.6
Oxidative phosphorylation and ATP synthesis
    orf19.3577 COQ5 Methyltransferase of ubiquinone (coenzyme Q) biosynthesis+1.5
    orf19.3008 COQ4 Ubiquinone biosynthesis+1.6
     COQ6 Ubiquinone biosynthesis−1.5
    orf19.7049 CYB5 Cytochrome b5+1.7
    orf19.2201 CBP6Cytochrome b protein synthesis+1.9
    orf19.1770 CYC1 Cytochrome c+2.0
    orf19.3527 CYT1 Cytochrome c1+1.6
    orf19.4578 CYT2 Cytochrome c1 heme lyase+1.8
    orf19.1416 COX11 Cytochrome c oxidase assembly+1.7
    orf19.2006.1 COX17Cytochrome c oxidase assembly+2.3
    orf19.3946 COX18Cytochrome c oxidase assembly+1.8
    orf19.4967 COX19 Required for cytochrome c oxidase activity+1.6‡
    orf19.2644 QCR2 Ubiquinol-cytochrome c reductase 40-kDa chain II+2.1
    orf19.5893 RIP1 Ubiquinol cytochrome c reductase+1.9
    orf19.4929 PET309Translational activator of COX1+1.6
    orf19.7577 MSS51 Required for translation of COX1+2.3
    orf19.6565 OXA1Assembly of cytochrome c oxidase, ATP synthase, and ubiquinol-cytochrome c oxidoreductase+2.2+1.4
    orf19.6854 ATP1 F1Fo ATPase complex+1.9
    orf19.5653 ATP2 F1Fo ATPase complex+2.3
    orf19.3579 ATP4 F1FoATPase complex+1.7
    orf19.5419 ATP5 F1Fo ATPase complex+1.8
    orf19.2785 ATP7 F1Fo ATPase complex+1.7‡+2.0
    CA4830* ATP8 F1Fo ATPase complex+1.4
    orf19.6916 ATP11F1Fo ATPase complex+2.0
    orf19.3686 ATP12 F1Fo ATPase complex+1.6‡
Lipid and fatty acid metabolism
    orf19.5577 YDR531WPantothenate kinase+1.7−1.7
    orf19.3171 ACH1 Acetyl-CoA hydrolase+2.0
    orf19.7466 ACC1 Acetyl-CoA carboxylase+1.9
    orf19.7043.1 ACB1 Acyl-CoA binding protein+1.4−1.6
ARE2 Acyl-CoA:sterol acyltransferase−2.8
    orf19.979 FAS1 Fatty acid synthase (beta subunit)+1.7
    orf19.5949 FAS2 Fatty acid synthase (alpha subunit)+1.5−1.6
    orf19.7592 FAA4 Long-chain-fatty acyl-CoA synthetase+1.4
    orf19.5818 SUR2 Hydroxylation of C-4 of sphingoid moiety of ceramide−1.9
    orf19.6860 PIS1 Phosphatidylinositol synthase+1.5
Ergosterol metabolism
    orf19.406 ERG1 Squalene epoxidase−1.9
    orf19.6026 ERG2 C-8 sterol isomerase+1.6
    orf19.5178 ERG5 C-22 sterol desaturase−1.5
    orf19.3616 ERG9 Farnesyl-diphosphate farnesyltransferase+1.3‡
    orf19.1598 ERG24 C-14 sterol reductase+1.3
    orf19.1591 ERG10 Acetyl-CoA acetyltransferase+1.7
    orf19.922 ERG11 Lanosterol 14-alpha-demethylase−2.4
    orf19.4631 ERG251 C-4 methylsterol oxidase activity−1.7
  • a The systematic (orf19) name from http://www.candidagenome.org is used where possible; one ORF was not represented in the Candida Genome Database, and the CandidaDB (http://genolist.pasteur.fr/CandidaDB/ ) designation was used (asterisk).

  • b The standard name from the Candida Genome Database is used where possible; where the standard name is the same as the systematic name, the name of the S. cerevisiae ortholog was substituted (†).

  • c —, changes in gene expression were not statistically reproducible with the methods used. This does not suggest that gene expression is unchanged.

  • d The genes with altered expression in yeast cells were mostly obtained using an FDR of 0.23%. The indicated genes (‡) were present only at an FDR of 0.99%.