TABLE 2.

Oligonucleotides used in this study

NameSequence
AgBOI1/2-S1AGGATGAGTTTTTCGTTCAAGAAGGCGCTGAAGCTGACCGGGATGGCTAGGGATAACAGGGTAAT
AgBOI1/2-S2TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAAGGCATGCAAGCTTAGATCT
AgBOI1/2-N1TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAGGGGCAGGGCATGCTCATGTAG
AgBOI1/2-N1TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAGGGGCAGGGCATGCTCATGTAG
AgBOI1/2-G1GAGGACTCTTGAAGGCACGG
AgBOI1/2-G4AATTGACTCCTAACGAAGCTCC
AgBOI1/2-IAACCAGGCCGATTCTGCGG
V2-NAT1GTGGTGAAGGACCCATCCAG
V3-NAT1ACATGAGCATGCCCTGCCCC
AgRHO3-N1CTGGCATCAGAGGAAGCTCCCACCACCAAGCTCTACAAACACAAGATGGGTACCACTCTTGACGAC
AgRHO3-N2ATATTAGTATAGTCTAAAGTTGCAGGCAGTGGGTATTAAAGTTCAGGGGCAGGGCATGCTCATGTAG
AgRHO3-G1CACGCGCAGCACGCGAGCTG
AgRHO3-G4CGCCGTTCCGCCTCGGCCGG
AgRHO3-ICTCCGAGGATGACGATCTTG
AgBOI1/2-GFP1TTATTTATTAATCTGGCAGGACTGCTCTTTCGTGTTTATAATACAAGGGACCTGGCACGGAGC
AgBOI1/2-GFP2AACGAGTACTATGGCCTAGATCCGAAATATATGGGTGAGAAGATTGGTGCAGGCGCTGGAGCTG
AgCYK1-N1GTAACGTATCGCTAGTTCAACCTACCAGCAGTGCATCGGGAGCTACTAGGATGGGTACCACTCTTGACGAC
AgCYK1-N2CGTATTAATTATTATTGTTGTCCTTATCCATCGTTACGTAAGTATAATTAGGGGCAGGGCATGCTCATGTAG
AgCYK-G1TAGAGACCACGGCATTTG
AgCYK-G4GGCTGCTTTCTCCTATTG
5SmaI-AgBOI1/2TCCCCCGGGTATGAGTTTTTCGTTCAAGAAGG
3BamHI-AgBOI1/2CGGGATCCTCAAATCTTCTCACCCATATATTTCG
5EcoRI-AgRHO3GATCGAATTCATGCCTCTGTGTGGGTCGAG
3BamHIAgRHO3CGACGGATCCTAACTGCTGCTTTCGGCCTCGG
AgRHO3_G24VforGTGGCTTGCGGGAAGACGTCGCTG
AgRHO3_(G24V)revGTCTCCGAGGATGACGATCTTG
AgRHO3_T29NforAACTCGCTGTTGAACGTGTTCAC
AgRHO3_(T29N)revCTTCCCGCAAGCACCGTCTCC
AgRHO3_Q71LforCTGCACATCACGCTGAGCCTGTG
AgRHO3_(Q71L)revGTTGTCCACGAAGATGTCATGG
AgBOI1/2ΔCforGCGAATGGCAATGCTAACGAGTAC
AgBOI1/2ΔCrevCTTACTCATCCAACCCGAGCACGC
AgBOI1/2ΔNforCGGAACGTTACAGTAAATGACGCTG
AgBOI1/2ΔNrevACTGTCGTCGCCCTGCTCCATCCCG