TABLE 1.

Oligonucleotide sequences

OligonucleotideSequence (5′ to 3′)
Als5SSf1ACAACTACCAACTGCTAACACCAGATG
Als5Igr1AGCAACAATGACAATACCGT
Als5TRr1AGAATCACTCGACTCAATA
Als5TCr1GGTACGAGTTGTAGTGGTAG
Als5EcoRI-Ig-5′CCGGAATTCGCGATCACTGGTATTTTC
Als5BglII-Ig-3′CCGAGATCTAGCAACAATGACAATAC
Als5BglII-ST-5′CCGAGA TCT GCTATTGAGTCGAGTGA
Als5XhoI-GPI-3′CCGCTCGAGTCATAGAAAGAAGAATAA
Als5BglII-His6X-TC-3CCGAGATCTATGATGATGATGATGATGTGGATTGGTACGAGTTG
Als5EcoRI-V5-5′CCGGAATTCGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCCAAATCCAACTACTACAAC
Als5BglII-TR-3′CCGAGATCTTGGATCATGTATAACGA
RD0329-1GTTGGTGTGACTACTTCCT
RD0329-2GAATCATCCAGTTACGTCT