TABLE 3.

Effect of Sla1ΔC expression on sporulation of various sporulation-defective mutants

Genotype% Sporulation with:
pREP1pREP1-Sla1FLpREP1-Sla1ΔC
byr2Δ/byr2Δa0015.3
byr1Δ/byr1Δa0017.6
spk1Δ/spk1Δa0011.0
phh1Δa009.8
mei3Δb0NTc22.2
mei2Δb0NT0
ste11Δb0NT0
  • a Each transformant was streaked onto a PM (+N) plate and incubated at 25°C for 72 h. Under these conditions wild-type cells sporulate more than 50%. The sporulation efficiency of at least 1,000 cells was then examined by counting the sporulating cells.

  • b Each transformant was streaked onto a PM(+N)-adenine (75 mg/liter) plate and incubated at 25°C for 72 h. The sporulation efficiency of at least 1,000 cells was then examined by counting the sporulating cells.

  • c NT, not tested.