TABLE 2.

Plasmids used in this study

PlasmidDescriptiona
pCLM814YEplac112-UASSLN1-SLN1-GFP
HOG1-GFPpRS416-HOG1-GFP (7)
pJL1363pRS425-UASSKN7-GFP
pJL1380pRS425-UASSKN7-GFP-SKN7
pJL1414pRS316-UASYPD1-GFPx2-YPD1
PJL1415PRS316-UASYPD1-GFPx2-ypd1H64Q
pJL1416pRS314-OCH1 (−336 to +26)-lacZ
pJL1419pRS315-UASYPD1-GFPx2-YPD1
PJL1429PRS416-UASYPD1-GFPx2-ypd1H64Q
pJL1433pRS315-UASYPD1-GFPx2-YPD1 H64Q
pJL1437pRS416-UASYPD1-GFPx2-YPD1-CaaX(CCIIS)
pJL1440pRS315-UASYPD1-GFPx2-YPD1-CaaX(CCIIS)
pJL1441pRS416-UASYPD1-GFPx2-YPD1-SaaX(SSIIS)
pJL1447pRS315-GFPx2-YPD1-SaaX(SSIIS)
pJL1453pRS316-UASYPD1-GFP-NLSx2-GFP-YPD1
pJL1454pRS315-UASYPD1-GFP-NLSx2GFP-YPD1
pJL1456pRS316-UASYPD1-GFPx2-NES-YPD1
pJL1461pRS315-UASYPD1-GFPx2-NES-YPD1
pJL1465pRS316-UASYPD1-GFPx2-SSK1
pJL1514pRS313-UASGAL10-GFP-NLSx2-GFP-YPD1
pJL1532pRS313-UASGAL10-GFP-HTB1
  • a All plasmids except HOG1-GFP (7) were constructed for this study. See Materials and Methods for construction details. Plasmids used solely as intermediates in various construction schemes are not listed here.