TABLE 1.

Fission yeast strains used in this study

StrainGenotypeSource
HS416RU h 90 ura4-D18 ade6-216 sam3 ras1::ura4+Lab stock
SP870 h 90 leu1-32 ura4-D18ade6-210 D. Beach
YM71 h leu1-32 ura4-D18 Lab stock
HM123 h leu1 Lab stock
SPRUDA h 90 leu1-32 ura4-D18 ade6-210 ras1::ura4+/h90leu1-32 ura4-D18 ade6-216 ras1::ura4+This study
SPRN1 h 90 leu1-32 ura4-D18 ade6-210 ras1BglII-NheID. Beach
SPRN1A h 90 leu1-32 ura4-D18 ade6-21 ras1BglII-NheID. Beach
SPRN1DA h 90 leu1-32 ura4-D18 ade6-210 ras1BglII-NheI/h90leu1-32 ura4-D18 ade6-216 ras1BglII-NheID. Beach
SPBUD h 90 leu1-32 ura4-D18 ade6-210 byr1::ura4+/h90leu1-32 ura4-D18 ade6-216 byr1::ura4+M. Wigler
SPSUD h 90 leu1-32 ura4-D18 ade6-210 byr2::ura4+/h90leu1-32 ura4-D18 ade6-216 byr2::ura4+M. Wigler
SPKUD h 90 leu1-32 ura4-D18 ade6-210 spk1::ura4+/h90leu1-32 ura4-D18 ade6-216 spk1::ura4+M. Wigler
TK105 h 90 leu1-32 ura4-D18 phh1::ura4+T. Kato
JZ127 h 90 leu1-32 ura4-D18 ade6-216 mei2::ura4+M. Yamamoto
JZ497 h 90 leu1-32 ura4-D18 ade6-216 mei3::ura4+M. Yamamoto
KJ33-1A h 90 leu1-32 ura4-D18 ade6-210 ste11::ura4+K. Kitamura
TW1 h 90 leu1-32 ura4-D18 ade6-210 sla1::ura4+This study
TW5 h 90 leu1-32 ura4-D18 ade6-210 ras1BglII-NheI sla1::ura4+/h90leu1-32 ura4-D18 ade6-216 ras1BglII-NheI sla1::ura4+This study
NOR 1 h leu1-32 ura4-D18 sla1 +-gfp<<LEU2aThis study
NOR 12 h 90 leu1-32 ura4-D18 sla1 +-gfp<<LEU2This study
S964 h leu1-32 ade6-210 pat1-114/h leu1-32 ade6-216 pat1-114 P. A. San-Segundo
HT148 h leu1-32 ura4-D18 ade6-216 pat1-114 Lab stock
KT101 h 90 leu1-32 ura4-D18 ade6-216 pat1-114 sla1::ura4+This study
KT102 h leu1-32 ura4-D18 ade6-216 pat1-114 sla1::ura4+This study
  • a sla1 +-gfp<<LEU2 indicates that LEU2 is close to sla1+-gfp.