TABLE 3.

Repression of ANB1-lacZ in SRB7 and/or HHF1 mutant strains

StrainaMean ± SD Miller units under the following conditions:Fold repressionb
AerobicAnaerobic
Wild type1.7 ± 1.490.1 ± 27.652.9
hhf1-8 6.9 ± 3.782.7 ± 22.712.0
srb7Δ73.4 ± 0.133.1 ± 3.29.7
Nub-srb7Δ73.5 ± 1.559.2 ± 22.916.8
srb7Δ7/hhf1-87.2 ± 0.880.5 ± 45.811.2
Nub-srb7Δ7/hhf1-813.3 ± 8.395.3 ± 54.57.2
  • a Enzyme assays were carried out by using MZ101-18 (wild type), MZ101-18A (hhf1-8), MZ101-18srb7Δ7N (srb7Δ7), MZ101-18Nub-srb7Δ7N (Nub-srb7Δ7), MZ101-18Asrb7Δ7N (srb7Δ7/hhf1-8), and MZ101-18ANub-srb7Δ7N (Nub-srb7Δ7/hhf1-8).

  • b β-Galactosidase activity in cells grown anaerobically for 2 h relative to that in cells grown aerobically.