TABLE 2.

Drug susceptibility of S. cerevisiae cells expressing the wild type and mutant variant of Cdr1p

StrainAmino acid changeMICa (μg/ml)
AnisoCyhFluMicNys
SS1K309E80.151620.5
SS2R308S160.0751620.5
SS3G305A160.153240.25
SS4G305V80.153240.5
SS5F774A160.037840.12
SS6ΔF77440.01840.50.03
SS7V773A160.153281
SS8V773I160.1532161
SS9D232E160.1564161
SS10D232K20.018410.03
SS11G296D80.03780.50.5
SS12G995S80.037880.5
SS13G998S320.07564321
SS14G1000C160.0751280.51
SS15T1351F0.50.00880.120.03
SS16C1418Y320.156411
SS17T1449I80.156410.5
SS18V1456I160.0756410.5
S6-2010.00420.250.015
AD-CDR1320.36481
  • a MICs were determined by a microdilution method as described in Materials and Methods. Aniso, anisomycin; Cyh, cycloheximide; Flu, fluconazole; Mic, miconazole; Nys, nystatin. The MIC test end point was defined as the MIC80.