TABLE 1.

Yeast strains used in this study

StrainaRelevant genotypeDerivation (reference)
JF1455 MAT ahis4-917 lys2-128δ trp1Δ1 ura3-52 leu2 sln1Δ::LEU2/SLN1Diploid strain
JF1565 MATα his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhRCanR CyhR derivative of FY834 (37)
JF1592 MAT a kar1-1 ade2-101 his4 leu2 trp1Δ1 or -62 ura3-52
JF1904 MATα skn7Δ::TRP1 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR skn7Δ::TRP1 derivative of JF1565; one-step replacement
JF1910b MATα sln1-22 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR sln1-22 derivative of JF1565; two-step replacement (36); plasmid free
JF1919 MATα ssk1Δ::LEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 ssk1Δ::LEU2 derivative of FY834; one-step replacement
JF1920b MATα sln1-22 ssk1Δ::LEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR ssk1Δ::LEU2 derivative of JF1910; one-step replacement
JF1974 MATα hog1Δ::TRP1 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR hog1Δ::TRP1 derivative of JF1565; one-step replacement
JF2123 MATα fps1Δ::LEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhRPlasmid-free version of JF1732 (36)
JF2148 MATα his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR; pRS426-PTP2JF1565 carrying pRS426-PTP2
JF2150 MATα ypd1Δ::kan ssk1Δ::LEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 ypd1Δ::kan derivative of JF1919; one-step replacement
JF2153 MATα ypd1Δ::kan his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR; pRS426-PTP2 ypd1Δ::kan derivative of JF2148; one-step replacement
JF2219b MATα sln1-22 ypd1Δ::kan ssk1Δ::LEU2 his3Δ200 leu2Δ1 ura3-52 trp1Δ63 lys2Δ201 canR cyhR ypd1Δ::kan derivative of JF1920; one-step replacement
  • a All strains used in this study except JF1455 were created by transformation of FY834 (37) or its canR cyhR derivative JF1565, both of which are isogenic to S288C. The JF1455 strain is congenic with S288C.

  • b sln1-22 is one of several activating alleles of SLN1 collectively referred to as sln1* alleles. The sln1-22 mutation causes a change of proline 1148 to serine (5).