TABLE 1.

Strains used in this study

StrainGenotypeStrain derivationReference
AD1234568MATa pdr1-3 his1 ura3 Δyor1::hisG Δsnq2::hisG Δpdr5::hisG Δpdr10::hisG Δpdr11::hisG Δycf1::hisG Δpdr15::hisGDecottignies et al. (9)
AD-CDR1AD1234568 transformant carrying cloned CDR1 with its native promoter in pYEURA3 (centromeric) vectorAD1234568This study
S6-20AD1234568 transformant carrying only the pYEURA3 (centromeric) vectorAD1234568This study
AD1-8uMATa pdr1-3 his1 ura3 Δyor1::hisG Δsnq2::hisG Δpdr5::hisG Δpdr10::hisG Δpdr11::hisG Δycf1::hisG Δpdr3::hisG Δpdr15::hisGNakamura et al. (33)
AD1002MATa pdr1-3 his1 ura3 Δyor1::hisG Δsnq2::hisG Δpdr5::PDR5PROM-CDR1-CDRISTOP Δpdr10::hisG Δpdr11::hisG Δycf1::hisG Δpdr3::hisG Δpdr15::hisGNakamura et al. (33)
PSCDR1-GFPMATa pdr1-3 his1 ura3 Δyor1::hisG Δsnq2::hisG Δpdr5::PDR5PROM-CDR1-GFP-GFPSTOP Δpdr10::hisG Δpdr11::hisG Δycf1::hisG Δpdr3::hisG Δpdr15::hisGAD1-8uThis study
SS1 to SS18AD1234568 transformant carrying cloned mutant CDR1 (SS1 to SS18 as described in the legend to Fig. 3b) with its native promoter in pYEURA3 (centromeric) vectorAD1234568This study
SS5G (F774A)MATa pdr1-3 his1 ura3 Δyor1::hisG Δsnq::hisG Δpdr5::PDR5PROM-CDR1(F774A)-GFP-GFPSTOP Δpdr10::hisG Δpdr11::hisG Δycf1::hisG Δpdr3::hisG Δpdr15::hisGAD1-8uThis study
SS6G (ΔF774)MATa pdr1-3 his1 ura3 Δyor1::hisG Δsnq::hisG Δpdr5::PDR5PROM-CDR1F774)-GFP-GFPSTOP Δpdr10::hisG Δpdr11::hisG Δycf1::hisG Δpdr3::hisG Δpdr15::hisGAD1-8uThis study