TABLE 2.

Primers used in this study

PrimerSequencea
3′ detecttgtggaattgtgagcggataacaatttcac
5′SnaBICaHis1gtagtggaagatattctttattgaaaaatagcttgtcaccggatcctggaggatgaggag
St. LacZ fus5′attaatgaacatgactgaaaaaattc
Mlu-Not-ACT1 UTRacgcgtgcggccgctttacaatcaaaggtggtcc
LacZ+354cacagcatttgattcttgagg
PHR2 ATGcatatggatcgaatgtgtgt
PHR2-1005cgtcgtgtggatcgagtggc
Pphr2-48m1gtttagtactttccttctagCCggcAAttcaaggaatcatcgtc
Pphr2-48m2gacgatgattccttgaaTTgccGGctagaaggaaagtactaaac
Pphr2-117m1cttttttttttcttgTTgccGGCtttcctccccttaactgg
Pphr2-117m2ccagttaaggggaggaaaGCCggcAAcaagaaaaaaaaaag
Pphr2-569m1gaaggaaaaaaaatatttcgccGGCttaaacatcgctggcgttg
Pphr2-569m2caacgccagcgatgtttaaGCCccggaaatattttttttccttc
PPHR2-117 aCCAAGA 5′ttcttcatctttcttttttttttcttgttTCTTGGttttcctccccttaactggttttttttct
PPHR2-117 aCCAAGA 3′agaaaaaaaaccagttaaggggaggaaaaCCAAGAaacaagaaaaaaaaaagaaagatgaagaa
PPHR2-117 GCCAAGg 5′ttcttcatctttcttttttttttcttgttcCTTGGCtttcctccccttaactggttttttttct
PPHR2-117 GCCAAGg 3′agaaaaaaaaccagttaaggggaggaaaGCCAAGgaacaagaaaaaaaaaagaaagatgaagaa
PPHE2-117 aCCAAGA 5′EMSAttttttttcttgttTCTTGGttttcctccccttaa
PPHE2-117 aCCAAGA 3′EMSAttaaggggaggaaaaCCAAGAaacaagaaaaaaaa
PPHR2-117 GCCAAGg 5′EMSAttttttttcttgttcCTTGGCtttcctccccttaa
PPHR2-117 GCCAAGg 3′EMSAttaaggggaggaaaGCCAAGgaacaagaaaaaaaa
BS-825shaaaaaaaaaCCAAGAAaaatattccatctttata
BS-825shctataaagatggaatatttTTCTTGGttttttttt
BS-825XshaaaaaaaaatCtAGAAaaatattccatctttata
BS-825XshctataaagatggaatatttTTCTaGattttttttt
PHR2-48 5a′agtactttccttctaCCAAGAAattcaaggaatca
PHR2-48 3a′tgattccttgaatTTCTTGGtagaaggaaagtact
PHR2-48mut 5a′agtactttccttctgCCggcAaattcaaggaatca
PHR2-48mut 3a′tgattccttgaattTgccGGcagaaggaaagtact
PHR2-117 5a′ttttttttcttgtTTCTTGGCtttcctccccttaa
PHR2-117 3a′ttaaggggaggaaaGCCAAGAAacaagaaaaaaaa
PHR2-117mut 5a′ttttttttcttgtTTgccGGCtttcctccccttaa
PHR2-117mut 3a′ttaaggggaggaaaGCCggcAAacaagaaaaaaaa
PHR2-569 5′gaaaaaaaatatttcCTTGGCttaaacatcgctgg
PHR2-569 3′ccagcgatgtttaaGCCAAGgaaatattttttttc
PHR2-569mut 5′gaaaaaaaatatttcgccGGCttaaacatcgctgg
PHR2-569mut 3′ccagcgatgtttaaGCCggcgaaatattttttttc
  • a Rim101 binding sites are in capital letters, and mutations are underlined.