TABLE 2.

Influence of Thr42 of Ste50p on the mating response of yeast with Δste20 or STE11 ΔSAM

Relevant genotypeMating efficiencya
Δste20 Δste50STE11ΔSAM Δssk2 Δssk22
Vector<0.1 × 10−53.0 × 10−5 ± 0.4 × 10−5
STE5034.0 × 10−5 ± 2.0 × 10−5450.0 × 10−5 ± 40.0 × 10−5
STE50-T42A1.0 × 10−5 ± 0.5 × 10−56.6 × 10−5 ± 0.5 × 10−5
STE50-T42D40.0 × 10−5 ± 2.0 × 10−5360.0 × 10−5 ± 30.0 × 10−5
  • a Mating efficiency is the number of diploids formed with the MATα tester strain DC17 cells divided by the number of input MATa cells in the mating mixture at the begining of the experiment multiplied by 100. The data are expressed as the averages of three repetitions, with standard deviations indicated. The strains used were YCW350 (MATa Δste20 Δste50) and YCW466 (MATa STE11ΔSAM Δssk2 Δssk22) transformed with the STE50 alleles indicated. All STE50 alleles were expressed as GST fusions from the CUP1 promoter from the 2μm plasmid.