TABLE 1.

Lhcb proteins identified by MS-MS data from protein spots excised from thylakoid 2-DE mapsa

2-DE SpotProteinbPeptidecPositionsdzeΔMXcorr
With FeWithout FeWith FeWithout Fe
12Lhca7 (p15)NPGSQADGSFLGFTEEFK141-15821.53.52
Lhca7 (p15)GLENGYPGGR159-16820.22.27
Lhca3 (p14.1)GSGDAAYPGGPFFNLFNLGK186-20521.34.10
Lhca3 (p14.1)GPFQNLVEHLADPVNNNILTNFGK241-26430.95.19
Lhca3 (p14.1)WLQYSEVIHAR86-9621.03.09
Lhcbm1VNGGPLGEGLDK158-16920.00.83.032.8
Lhcbm1QPLQNLSDHLANPGTNNAFAYATK228-25131.56.31
Lhcbm1, 10ASTPDSFWYGPER20.91.12.722.37
Lhcbm1-10YRELELIHAR3 (2)1.10.83.603.48
13Lhca7 (p15)VGLGFPEWYDAGK95-10720.13.41
Lhca3 (p14.1)GSGDAAYPGGPFFNLFNLGK186-2052−0.63.85
Lhca3 (p14.1)GPFQNLVEHLADPVNNNILTNFGK241-2643−0.96.29
Lhca3 (p14.1)WLQYSEVIHAR86-9620.84.18
Lhcbm1LYPGGSFDPLGLADDPDTFAELKVK170-19420.42.36
Lhcbm1VNGGPLGEGLDK158-1692−0.20.03.162.84
Lhcbm1GPLQNLSDHLANPGTNNAFAYATK229-25231.36.40
Lhcbm1, 10ASTPDSFWYGPER21.01.12.572.48
Lhcbm1-10YRELELIHAR30.84.09
Lhcbm1-10ELELIHAR20.92.59
14Lhcbm1VNGGPLGEGLDK158-16920.8−0.23.123.42
Lhcbm1LYPGGSFDPLGLADDPDTFAELKVK170-19431.43.84
Lhcbm1, 10ASTPDSFWYGPER21.00.92.783.46
Lhcbm2, 8ANGGPLGEGLDPLHPGGAFDPLGLADDPD TFAELK160-19431.24.02
Lhcbm1-10YRELELIHAR31.04.35
Lhcbm1-10ELELIHAR20.72.58
15Lhcbm1VNGGPLGEGLDK158-16920.4−0.32.653.28
Lhcbm2, 8IEWYGPDRPK30-3920.80.92.712.31
Lhcbm2, 8NGIPFGEAVWFK105-11620.20.03.112.48
Lhcbm1-10ELELIHAR21.12.35
16Lhcbm1VNGGPLGEGLDK158-16920.72.98
Lhcbm1-10YRELELIHAR20.73.51
Lhcbm1-10ELELIHAR20.82.28
Lhcbm2, 8IEWYGPDRPK30-3920.90.32.502.48
Lhcbm2, 8NGIPFGEAVWFK105-1162−0.1−0.42.632.83
Lhcbm2, 8GPIQNLDDHLANPTAVNAFAYATK221-24431.45.76
Lhcbm2, 8FLGPFSEGDTPAYLTGEFPGDYGWDTAGLS ADP ETFKR40-7741.14.24
Lhcbm2, 3, 4, 8, 10WAMLGALGCL(I)TPELLAK21.44.87
Lhcbm6WAMLGALGCQTPELLAK92-10820.20.22.252.40
17Lhcbm1VNGGPLGEGLDK158-16920.60.92.663.04
Lhcbm1, 10ASTPDSFWYGPER21.02.98
Lhcbm1-10YRELELIHAR3 (2)−0.30.93.173.29
Lhcbm1-10ELELIHAR2−0.10.72.252.35
Lhcbm2, 8IEWYGPDRPK30-3920.30.82.662.35
Lhcbm2, 3, 4, 5, 8, 10WAMLGALGCL(I)TPELLAK21.34.87
Lhcbm2, 8FLGPFSEGDTPAYLTGEFPGDYGWDTAGL SADPETFK40-7630.66.66
Lhcbm2, 8FLGPFSEGDTPAYLTGEFPGDYGWDTAGL SADP ETFKR40-773−1.24.48
Lhcbm2, 8NGIPFGEAVWFK105-1162−0.42.99
Lhcbm2, 8ANGGPLGEGLDPLHPGGAFDPLGLADDP DTFA ELK160-19430.44.61
Lhcbm2, 8GPIQNLDDHLANPTAVNAFAYATK221-2443−0.61.35.276.50
Lhcbm6WAMLGALGCQTPELLAK92-10820.32.35
22Lhcbm1-10YRELELIHAR3−1.23.68
Lhcbm1-10ELELIHAR20.8−0.12.272.39/PICK>
Lhcbm2, 3, 4, 5, 8, 10WAMLGALGCL(I)TPELLAK20.95.24
Lhcbm3QAPASSGIEFYGPNR31-4521.00.53.643.43
Lhcbm3, 6WLGPYSENATPAYLTGEFPGDYGWDTAGL SADPETFKR30.44.91
Lhcbm3, 7, 10GPIQNLDDHLSNPTVNNAFAFATK30.15.07
Lhcbm4, 6FGEAVWFK20.41.03.563.35
Lhcbm4, 6SSGVEFYGPNR21.0−0.72.492.43
Lhcbm3, 4, 6, 10SGTK(Q)FGEAVWFK20.42.97
Lhcbm4, 6, 7, 9, 10VNGGPAGEGLDPLYPGESFDPLGLADDPDT FAELK3−1.04.30
Lhcbm6GPVQNLDDHLANPTVNNAFAFATK222-24530.05.41
Lhcbm6WAMLGALGCQTPELLAK92-10820.13.01
23Lhcbm1-10YRELELIHAR2−0.23.90
Lhcbm1-10ELELIHAR20.90.22.562.76
Lhcbm2, 3, 4, 5, 8, 10WAMLGALGCL(I)TPELLAK20.84.79
Lhcbm3QAPASSGIEFYGPNR31-4520.9−0.83.443.40
Lhcbm3SSGIEFYGPNR35-4520.90.33.072.28
Lhcbm3, 6WLGPYSENATPAYLTGEFPGDYGWDTAGL SADPETFKR31.34.02
Lhcbm3, 7, 10GPIQNLDDHLSNPTVNNAFAFATK30.0−0.24.676.34
Lhcbm4, 6FGEAVWFK20.90.23.38
Lhcbm4, 6SSGVEFYGPNR20.8−0.52.372.80
Lhcbm3, 4, 6, 10SGTK(Q)FGEAVWFK20.43.37
Lhcbm4, 6, 7, 9, 10VNGGPAGEGLDPLYPGESFDPLGLADDPD TFAELK30.13.42
Lhcbm6GPVQNLDDHLANPTVNNAFAFATK222-2453−1.35.95
24Lhcbm1-10ELELIHAR21.00.42.712.49
Lhcbm1VNGGPLGEGLDK158-16920.42.77
Lhcbm1-10ASTPDSFWYGPER20.53.27
Lhcbm3, 7, 10GPIQNLDDHLSNPTVNNAFAFATK30.70.95.385.68
Lhcbm3QAPASSGIEFYGPNR31-4521.0−0.93.503.53
Lhcbm3SSGIEFYGPNR35-4521.22.26
Lhcbm4, 6SSGVEFYGPNR20.9−0.82.282.65
Lhcbm3, 4, 6, 10SGTK(Q)FGEAVWFK20.63.20
30fCP29GSVEAIVQATPDEVSSENR102-1202−0.74.67
CP29FADGLNNGK270-27820.02.94
31fCP26GWLGGQGGAADLDK38-5120.94.23
CP26KLFLPSGLYDR58-5620.92.91
CP26YRENELLHAR100-10920.74.07
CP26WAMLAAAGILIPEGLQANGANIK110-1323−0.14.79
CP26NGTGPAGYSPGIGK185-1982−0.23.04
CP26HVADPFGYNLLTVLGAEER267-28521.32.55
  • a The charge state of the measured ion (z), the calculated deviation of the experimentally determined mass from the theoretical average mass of the peptide (ΔM), and the Xcorr calculated by using the Sequest algorithm are shown. All peptide sequences reported produced Xcorr values equal to or above 1.5, 2.25, or 3.5 for singly, doubly, or triply charged precursor ions, respectively.

  • b The peptide ions identified as lhcb gene products are organized according to the 2-DE protein spots from which they originated (Fig. 1). The gene product and SDS-PAGE mobility identifier are shown. To conserve space in the table, protein designations were shortened; for example, “Lhcbm1, 10” indicates Lhcbm1 and Lhcbm10, and “Lhcbm1-10” indicates Lhcbm1 to Lhcbm10.

  • c The peptide sequence retrieved by database searching is shown.

  • d Positions of identified peptides within the database protein sequence.

  • e Values shown are for iron-sufficient (iron-deficient) conditions.

  • f Present in iron-sufficient and iron-deficient conditions; measured for iron-deficient conditions only.