TABLE 2.

Lhca proteins identified by MS-MS data from protein spots excised from PSI 2-DE mapsa

2-DE spotProteinbPeptidecPositionsdzΔMXcorr
1Lhca9 (p22.2)GALAGDNGFDPLGLGQDEGR43-6220.14.81
Lhca9 (p22.2)WYAEAEK65-711−0.61.77
2Lhca1 (p22.1)RFTESEVIHGR73-832−0.14.09
Lhca1 (p22.1)GDAGGVVYPGGAFDPLGFAK146-1652−0.34.04
Lhca1 (p22.1)FTESEVIHGR74-831−0.63.45
Lhca9 (p22.2)PTWLPGLNPPAHLK29-4230.03.52
3Lhca5 (p15.1)GDLAGDYGWDPLGLGADPTALK47-6820.64.88
Lhca5 (p15.1)VPNPEMGYPGGIFDPFGFSK157-1762−0.63.68
Lhca5 (p15.1)NFGSVNEDPIFK142-1532−0.13.65
Lhca5 (p15.1)LWAPGVVAPEYLK34-462−0.13.02
Lhca3 (p14.1)GPFQNLVEHLADPVNNNILTNFGK241-26431.25.95
Lhca3 (p14.1)GSGDAAYPGGPFFNLFNLGK186-2052−0.63.95
Lhca3 (p14.1)WLQYSEVIHAR86-962−0.23.81
Lhca7 (p15)VGLGFPEWYDAGK95-10720.03.37
Lhca7 (p15)GLENGYPGGR159-1682−0.33.15
Lhca7 (p15)YQEYK183-1871−0.51.97
4Lhca3 (p14.1)WLQYSEVIHAR86-962−0.63.80
Lhca3 (p14.1)WLQYSEVIHAR86-9630.03.56
Lhca3 (p14.1)TEAAMK206-2111−0.41.78
Lhca7 (p15)VGLGFPEWYDAGK95-10720.43.35
Lhcbm5LSAFYGPDR49-5720.32.66
5Lhca3 (p14.1)WLQYSEVIHAR86-962−0.24.08
Lhca3 (p14.1)WLQYSEVIHAR86-9630.33.57
6Lhca5 (p15.1)GDLAGDYGWDPLGLGADPTALK47-682−1.24.96
Lhca5 (p15.1)NFGSVNEDPIFK142-1532−0.33.89
Lhca7 (p15)VGLGFPEWYDAGK95-1072−0.23.47
Lhca7 (p15)YQEYK183-1871−0.41.72
Lhca7 (p15)WYDFK136-1401−0.41.55
7Lhca5 (p15.1)GDLAGDYGWDPLGLGADPTALK47-682−0.85.06
Lhca5 (p15.1)NFGSVNEDPIFK142-15320.74.28
Lhca5 (p15.1)VPNPEMGYPGGIFDPFGFSK157-1762−0.84.01
Lhca5 (p15.1)LWAPGVVAPEYLK34-4620.93.12
Lhca6 (p18.1)GDLPGDFGFDPLGLGANAESLK44-652−0.84.47
Lhca6 (p18.1)KPGSVDQDPIFSQYK134-1482−0.54.07
Lhca6 (p18.1)IPASVSYK235-2421−0.51.86
Lhca7 (p15)VGLGFPEWYDAGK95-1072−0.63.20
Lhca4 (p14)WYAQAELMNAR95-1052−0.52.82
8Lhca2 (p19)ESVPYFPWNEPWNKV232-2462−0.73.09
Lhca2 (p19)LGVNKDNLK56-642−0.42.77
Lhca2 (p19)ESVPYFPWNEPWNK232-2452−0.62.52
Lhca2 (p19) or Lhca10 (p18.2)YEIYKK126-13120.02.22
Lhca2 (p19) or Lhca10 (p18.2)YEIYK126-1301−0.51.59
Lhca2 (p19)SEEMK150-15410.01.50
Lhca2 (p19) or Lhca10 (p18.2)FWTAGAEK95-1021−0.61.70
Lhca2 (p19)TLNPGK226-2311−0.21.59
Lhca5 (p15.1)NFGSVNEDPIFK142-1532−0.54.16
Lhca7 (p15)VGLGFPEWYDAGK95-1072−0.23.61
9Lhca5 (p15.1)GDLAGDYGWDPLGLGADPTALK47-682−0.45.69
Lhca5 (p15.1)NFGSVNEDIFK142-1532−0.33.79
Lhca5 (p15.1)LWAPGVVAPEYLK34-462−0.53.17
Lhca6 (p18.1)KPGSVDQDPIFSQYK134-1482−0.35.03
Lhca6 (p18.1)GDLPGDFGFDPLGLGANAESLK44-652−1.03.89
Lhca6 (p18.1)IPASVSYK235-24210.71.88
Lhca2 (p19)TGETGFLSFAPFDPM[+16]GMK132-14920.04.07
Lhca10 (p18.2)TGETGFLSYAPFDPMGMK20.02.61
Lhca2 (p19)ESVPYFPWNEPWNKV232-2462−0.82.59
Lhca2 (p19) or Lhca10 (18.2)FWTAGAEK95-1021−0.62.06
Lhca2 (p19)LGVNKDNLK56-6420.03.41
Lhca2 (p19)TLNPGK226-2311−0.41.59
10Lhca5 (p15.1)KLWAPGVVAPEYLK33-462−0.15.00
Lhca5 (p15.1)VPNPEMGYPGGIFDPFGFSK157-1762−0.84.58/PICK>
Lhca5 (p15.1)GDLAGDYGWDPLGLGADPTALK47-682−1.04.51
Lhca5 (p15.1)NFGSVNEDPIFK142-1532−0.64.01
Lhca5 (p15.1)LWAPGVVAPEYLK34-462−0.53.06
Lhca6 (p18.1)KPGSVDQDPIFSQYK134-1482−0.54.1
Lhca6 (p18.1)AAVVPGQAVAPPCK221-23420.22.82
Lhca6 (p18.1)IPASVSYK235-2421−0.71.59
11Lhca6 (p18.1)KPGSVDQDPIFSQYK134-1482−0.34.42
Lhca6 (p18.1)GDLPGDFGFDPLGLGANAESLK44-652−0.44.24
Lhca6 (p18.1)KPGSVDQDPIFSQYK134-1483−0.14.10
Lhca6 (p18.1)ESELVHSR69-762−0.22.45
Lhca6 (p18.1)IPASVSYK235-2421−0.62.06
25Lhca8 (p18)WYQQAELIHCR68-782−0.44.24
Lhca8 (p18)YATGAGPVDNLAAHLK211-2262−0.24.04
Lhca8 (p18)GTSELGYPGGPFDPLGLSK162-18020.13.99
Lhca8 (p18)AGALNVPEWYDAGK96-1092−0.23.36
Lhca8 (p18)EADKWADWK181-1892−0.42.35
Lhca8 (p18)WADWK185-1891−0.41.60
Lhca8 (p18)KPGSQGEPGSFLGFEASLK144-1612−0.65.15
26Lhca7 (p15)NPGSQADGSFLGFTEEFK141-1582−0.55.12
Lhca7 (p15)VGLGFPEWYDAGK95-1072−0.43.40
Lhca7 (p15)GLENGYPGGR159-16820.32.67
Lhca7 (p15)GLENGYPGGR159-1682−0.12.51
Lhca7 (p15)FFDPMGLSR169-1772−0.42.36
Lhca7 (p15)YQEYK183-1871−0.41.83
Lhca7 (p15)VVVEK108-1121−0.31.52
29Lhca1 (p22.1)GDAGGVVYPGGAFDPLGFAK146-1652−0.74.11
Lhca1 (p22.1)FTESEVIHGR74-832−0.33.57
30Lhca9 (p22.2)GALAGDNGFDPLGLGQDEGR43-622−0.14.08
Lhca1 (p22.1)GDAGGVVYPGGAFDPLGFAK146-1652−0.63.95
31Lhca9 (p22.2)GALAGDNGFDPLGLGQDEGR43-622−0.36.01
Lhca9 (p22.2)TQPIEGLTAHLADPFGK181-19720.03.70
Lhca9 (p22.2)WYAEAEK65-711−0.61.60
2+Lhca1 (p22.1)GDAGGVVYPGGAFDPLGFAK146-1652−0.53.71
Lhca1 (p22.1)FTESEVIHGR74-832−0.33.33
Lhca9 (p22.2)GALAGDNGFDPLGLGQDEGR43-622−0.13.24
29+Lhca1 (p22.1)GDAGGVVYPGGAFDPLGFAK146-1652−0.14.18
Lhca1 (p22.1)FTESEVIHGR74-832−0.13.29
  • a The charge state of the measured ion (z), the calculated deviation of the experimentally determined mass from the theoretical average mass of the peptide (ΔM), and the Xcorr calculated by using the Sequest algorithm are shown. All peptide sequences reported produced Xcorr values equal to or above 1.5, 2.25, or 3.5 for singly, doubly, or triply charged precursor ions, respectively.

  • b The peptide ions identified as lhca gene products are organized according to the PSI 2-DE protein spots from which they originated. The gene product and SDS-PAGE mobility identifier are shown.

  • c The peptide sequence retrieved by database searching is shown.

  • d Positions of identified peptides within the database protein sequence.