TABLE 2.

Effects of the absence of Ppz1 and Ppz2 functions on the activities of the FRE1, FIT2, SIT1, and TRR1 promoters

Reporter and backgroundβ-Galactosidase activity (fold induction)a in the following mutant:
Wild typeppz1ppz2ppz1 ppz2
pFRE1
    JA1004.4 ± 0.3 (1.0)4.1 ± 0.8 (0.9 ± 0.2)3.0 ± 0.4 (0.7 ± 0.1)14.8 ± 1.6 (3.4 ± 0.4)
    DBY7461.7 ± 0.1 (1.0)1.3 ± 0.2 (0.8 ± 0.1)1.3 ± 0.2 (0.7 ± 0.1)27.9 ± 8.7 (16.6 ± 2.2)
pFIT2
    JA1004.2 ± 0.5 (1.0)3.2 ± 0.2 (0.8 ± 0.0)2.1 ± 0.2 (0.8 ± 0.1)41.3 ± 3.0 (9.9 ± 0.7)
    DBY7460.6 ± 0.1 (1.0)1.1 ± 0.3 (1.9 ± 0.5)1.0 ± 0.1 (1.8 ± 0.2)47.8 ± 5.1 (83.1 ± 8.8)
pSIT1
    JA100166.6 ± 9.9 (1.0)128.3 ± 11.8 (0.8 ± 0.1)140.0 ± 6.0 (0.8 ± 0.0)350.4 ± 25.4 (2.1 ± 0.2)
    DBY746174.4 ± 6.9 (1.0)185.4 ± 7.4 (1.1 ± 0.0)179.0 ± 5.3 (1.0 ± 0.0)627.1 ± 35.7 (3.6 ± 0.2)
pTRR1
    JA100111.1 ± 9.9 (1.0)132.7 ± 9.1 (1.2 ± 0.1)118.8 ± 2.6 (1.1 ± 0.0)424.2 ± 39.6 (3.8 ± 0.4)
    DBY746164.5 ± 6.0 (1.0)148.6 ± 9.2 (0.9 ± 0.1)151.7 ± 3.9 (0.9 ± 0.0)336.0 ± 21.1 (2.0 ± 0.1)
  • a β-Galactosidase activity is expressed in Miller units. Fold induction is given relative to activity in the isogenic wild-type strain.