TABLE 1.

S. cerevisiae strains used in this study

StrainRelevant genotypeaSource
SO329MATaFUS1::lacZ::LEU234
SO330MATaFUS1::lacZ::leu2 hog1::hisG34
PW014MATaFUS1::lacZ::LEU2 hog1::URA3This study
PW017MATaFUS1::lacZ::LEU2 hog1-asThis study
PW158MATaFUS1::lacZ::LEU2 hog1(K52R)This study
PW347MATaFUS1::lacZ::LEU2 hog1(D144A)This study
PW245MATaFUS1::lacZ::LEU2 HOG1-GFP-TRP1This study
PW246MATaFUS1::lacZ::LEU2 hog1-as-GFP-TRP1This study
PW247MATaFUS1::lacZ::LEU2 hog1(K52R)-GFP-TRP1This study
PW334MATaFUS1::lacZ::LEU2 hog1-as-GFP-TRP1 Nmd5-mCherry-URA3This study
PW348MATaFUS1::lacZ::LEU2 hog1(D144A)-GFP-KanrThis study
PW349MATaFUS1::lacZ::LEU2 HOG-GFP-TRP1 nmd5::URA3This study
PW287MATaTEC1::lacZ::URA3 TEC1This study
PW288MATaTEC1::lacZ::URA3 TEC1 hog1::TRP1This study
PW289MATaTEC1::lacZ::URA3 TEC1 hog1-asThis study
PW290MATaTEC1::lacZ::URA3 TEC1 hog1(K52R)This study
PW356MATaTEC1::lacZ::URA3 TEC1 hog1(D144A)This study
PW322MATa/MATα TEC1::lacZ::URA3 TEC1/TEC1This study
PW323MATa/MATα TEC1::lacZ::URA3 TEC1/TEC1 hog1::TRP1/hog1::TRP1This study
PW324MATa/MATα TEC1::lacZ::URA3 TEC1/TEC1 hog1-as/hog1-asThis study
PW325MATa/MATα TEC1::lacZ::URA3 TEC1/TEC1 hog1(K52R)s/hog1(K52R)This study
PW325MATa/MATα TEC1::lacZ::URA3 TEC1/TEC1 hog1(K52R)s/hog1(K52R)This study
PW419MATa/MATα TEC1::lacZ::URA3 TEC1/TEC1 hog1(D144A)/hog1(D144A)This study
PW483MATaFUS1::lacZ::LEU2 [YEp352-GAL (URA3)]This study
PW484MATaFUS1::lacZ::leu2 hog1::hisG [YEp352-GAL (URA3)]This study
PW485MATaFUS1::lacZ::LEU2 hog1-as [YEp352-GAL (URA3)]This study
PW486MATaFUS1::lacZ::LEU2 [YEp352-GAL-ssk2ΔN (URA3)]This study
PW487MATaFUS1::lacZ::leu2 hog1::hisG [YEp352-GAL-ssk2ΔN (URA3)]This study
PW488MATaFUS1::lacZ::LEU2 hog1-as [YEp352-GAL-ssk2ΔN (URA3)]This study
PW534MATahog1-as-GFP-TRP1 NUP1-mCherry-URA3This study
PW535MATaFUS1::lacZ::LEU2 HOG1-GFP-Kanrnmd5::TRP1 nup133::HIS4 NUP1-mCherry-URA3This study
  • a Strains PW014 to PW349, PW534, and PW535 are in the EG123 background, whose full genotype is trp1 leu2 ura3 his4 can1. Strains PW287 to PW419 are in the Σ1278b background, whose full genotype is trp1 leu2 ura3 his3.