TABLE 1.

Correlative peptide matches for proteins immunoprecipitated with MAb 28G2 and analyzed by LC-MS/MSa

Gel bandProtein prediction or namePeptidePosition
p240Chr11.genfinder_174rK.NPIDNSNISNLDK121-133
K.NVPHLDQSAMSNEK220-233
K.LALVPFQGIK560-569
K.EGPIITPLEGEQAGTAHK612-629
R.IIIEIMESAK768-777
K.DKNLISLEVYDK813-824
K.ILTEMSFYEDSK832-843
K.FYETLGIK844-851
K.YYQNMLGFEEDK947-958
K.VSSVPFNYENVK1008-1019
K.PSSSIIGSLGNLIK1021-1034
R.INSYFHYTEK1048-1057
K.IISVCTLLHLTDMLYK1067-1082
K.MVLQYLVHLK1108-1117
K.EICEPQNGLIDETLTK1129-1144
K.MLILLSTDSHELLSHELENK1145-1164
K.GFDEDYIQDEIK1165-1176
p85PfAMA1G.QNYWEHPYQNSDVY*25-38
R.SNYMGNPWTEYMAK103-116
K.YDIEEVHGSGIR117-128
R.VDLGEDAEVAGTQYR129-143
K.GIIIENSNTTFLTPVATGNQYLK155-177
K.NLDELTLCSR210-219
R.HAGNMIPDNDKNSNYK220-235
K.YPAVYDDKDK236-245
K.DISFQNYTYLSK281-292
K.LVFELSASDQPK340-351
R.AEVTSNNEVVVK514-525
K.DEYADIPEHKPTYDK530-544
I.ASSAAVAVLATILMVYLYK**550-568
R.NDEMLDPEASFWGEEK592-607
p70PfAMA1R.SNYMGNPWTEYMAK103-116
R.VDLGEDAEVAGTQYR129-143
K.DISFQNYTYLSK281-292
R.NDEMLDPEASFWGEEK592-607
  • a Column 1 is the designation of the Coomassie-stained band as indicated in Fig. 1A. Column 2 indicates the protein to which the identified peptides map. Column 3 lists the individual peptide sequences identified. All of the spectra were manually examined for ion coverage and signal-to-noise ratios. The predicted upstream amino acid is shown to the left of the period. Column 4 indicates the location of the peptide in each of the protein sequences. A single asterisk indicates the predicted N terminus of PfAMA183, and double asterisks indicate a peptide consistent with a tryptic fragment where not all residues were identified.