TABLE 2.

Virulence-related genes down regulated during heat shocka

OligonucleotideNCBI accession no.bIdentificationInhibition (fold)c
EH-IP0234.1 17.t00056 Gal/GalNAc lectin 35 subunit Lgl-17.4
EH-IP0234.2 17.t00056 Gal/GalNAc lectin 35 subunit Lgl-16.5
EH-IP0234.3 17.t00056 Gal/GalNAc lectin 35 subunit Lgl-11.9
EH-IP0816 56.t00038 Gal/GalNAc lectin 35 subunit Lgl-32.5
EH-IP0796.1 53.t00006 Gal/GalNAc lectin subunit Igl11.8
EH-IP0796.2 53.t00006 Gal/GalNAc lectin subunit Igl12.2
EH-IP0100 119.t00005 Gal/GalNAc lectin subunit Igl26.6
EH-IP0491.2 L14815 d Gal/GalNAc lectin subunit Hgl-32.4
EH-IP0715 442.t00004 Peroxiredoxin7.6
EH-IP0160 136.t00007 Peroxiredoxin18.6
EH-IP1002 963.t00001 Peroxiredoxin16.7
EH-IP0928.2 79.t00025 Cysteine protease 17.9
EH-IP0928.1 79.t00025 Cysteine protease 19.1
EH-IP0409 242.t00001 Cysteine protease 17.5
EH-IP0343.2 206.t00005 Cysteine proteinase 27.1
EH-IP0343.1 206.t00005 Cysteine proteinase 27.1
EH-IP0343.3 206.t00005 Cysteine proteinase 27.5
EH-IP0343.6 206.t00005 Cysteine proteinase 26.3
EH-IP0343.4 206.t00005 Cysteine proteinase 26.1
EH-IP0343.5 206.t00005 Cysteine proteinase 26.9
EH-IP1066 X87214 e Cysteine protease 31.3
EH-IP0626.2 358.t00002 Cysteine protease 86.0
EH-IP0626.1 358.t00002 Cysteine protease 87.6
EH-IP1070 AY156071 f Cysteine protease 132.6
EH-IP0261 180.t00007 Cysteine protease 171.7
EH-IP0288.2 191.t00018 Cysteine proteinase1.2
EH-IP0254.1 18.t00027 Amoebapore C7.5
EH-IP0254.2 18.t00027 Amoebapore C5.2
EH-IP0149.2 132.t00016 20-kDa antigen1.7
EH-IP0149.1 132.t00016 20-kDa antigen1.4
EH-IP0216 160.t00008 Surface antigen ariel117.4
EH-IP0614 349.t00010 Galactokinase16.4
  • a Notice that the surface molecule ARIEL (an asparagine-rich E. histolytica antigen of unknown function) (12) was down regulated 17-fold by heat shock, whereas amoebapore C was down regulated fivefold. Galactokinase (with a 16-fold down regulation) catalyzes phosphorylation of galactose during its catabolism. The enzyme has attracted significant research attention because of its important metabolic role, notably in humans, where defects in the enzyme can result in the disease state referred to as galactosemia (6). In addition, galactokinase-like molecules have been shown to act as sensors for the intracellular galactose concentration and, under suitable conditions, to function as transcriptional regulators (17).

  • b http://www.ncbi.nlm.nih.gov/gquery/gquery.fcgi?term=ENTAMOEBA.

  • c Rate of inhibition of gene transcription after heat shock.

  • d Hgl-3 allele published in reference 15.

  • e CP3 sequence published in reference 4.

  • f CP13 sequence published in reference 4.