Genotypes of the progeny of daughter cells from a cross between strains WO-1 (MTLα) and P37005 (MTLa)a

Parent strain or daughter cellIsolatesMTL mating typeBand
    P37005 a +++
Daughter cells
    3-131-12 a +++
    3-161-12 a +++
    4-61-12 a +++
    4-57, 9, 12 a +++
1-6, 8, 10, 11α+++
  • a Daughter cells from fusions were picked with a micromanipulator and grown; 12 cells from each clone were in turn cloned, grown, and analyzed by Southern blot hybridization. Cells were analyzed for mating type and for the presence (+) of the two NIK1 bands and the two URA3 bands (Fig. 9).