TABLE 3.

Overexpression of TdCRZ1 permits the calcineurin-dependent induction of a 4x CDRE-lacZ gene fusion in response to Ca2+

Relevant genotype (+ growth condition) of S. cerevisiae strain usedaβ-Galactosidase activity (U/mg of protein)b
ControlCa2+
Wild type152.7 ± 43.21,336.6 ± 98.1
crz1Δ0.9 ± 0.31.8 ± 0.4
crz1Δ TdCRZ157.0 ± 4.0322.4 ± 19.8
crz1Δ CRZ185.0 ± 7.0246.1 ± 23.2
cnb1Δ crz1Δ1.7 ± 0.24.2 ± 1.3
cnb1Δ crz1Δ TdCRZ1121.0 ± 14.2184.6 ± 12.2
cnb1Δ crz1Δ CRZ167.8 ± 8.4110.5 ± 17.1
crz1Δ TdCRZ1 + FK506 (1 μg/ml)52.0 ± 12.0157.4 ± 31.0
crz1Δ TdCRZ1 + FK506 (5 μg/ml)57.0 ± 21.060.4 ± 18.0
  • a The S. cerevisiae strains used were YPH499 (wild type), ASY834 (crz1Δ), and ASY835 (cnb1Δ crz1Δ) transformed with plasmid YEplac195 (empty plasmid), YEpTdCRZ1 (TdCRZ1), or pAMS435 (CRZ1).

  • b Data are means ± standard errors for three independent experiments. β-Galactosidase activities are shown for the indicated strains treated with 0.2 M CaCl2 in the presence or absence of FK506.