TABLE 1.

Identification of phosphopeptides from beta-casein, which were added to the total phosphopeptide sample from C. reinhardtii, by LC-ESI-MS2 and neutral-loss-triggered MS3

Phosphopeptide sequenceaNCBI accession no.MS typezbXcorr
FQS*EEQQQTEDELQDKgi|30794310
FQSpEEQQQTEDELQDKMS225.20
FQ(Dha)EEQQQTEDELQDKMS325.44
RELEELNVPGEIVES*LS*S*S*EESITRgi|30794310
RELEELNVPGEIVESpLSp(Dha)(Dha)EESITRMS335.60
RELEELNVPGEIVESpL(Dha)(Dha)SpEESITRMS335.55
  • a *, known phosphorylation sites within the beta-casein phosphopeptides (15); p, phosphorylated residue; Dha, dehydroalanine.

  • b z, charge.