TABLE 2.

Two-hybrid interactions of Rga1, Rga2, and Bem3, with Cdc42 and Rsr1a

PlasmidLevel of interaction with products of:
Original plasmidRga1 hybridRga2 hybridBem3 hybrid
pEG2026646
pEG202-CDC421211
pEG202-CDC42C188S1813545
pEG202-CDC42G12V,C188S1432,5761,3271,303
pEG202-CDC42Q6IL,C188S1372,4421,3991,324
pEG202-CDC42D118A,C188S1111
pEG202-RSR1196551
pEG202-RSR1G12V184561
pJG4-59111
pJG4-5-CDC426112
pJG4-5-CDC42C188S3111
pJG4-5-CDC42G12V,C188S2100106
pJG4-5-CDC42Q61L,C188S210986
  • a Products of plasmid pEG202 and its constructs interacted with products of pJG4-5 and its constructs and vice versa. β-Galactosidase activity was determined as described in Materials and Methods. The reported values are given in Miller units and are an average of three independent determinations.