TABLE 6.

Activation of FUS1-lacZ by deletion of GAPs for Cdc42

StrainGenotypeaFUS1-lacZ expressionb
YGS58 ste4Δ1.2 ± 0.1
YGS59 ste4Δrga1Δ38.9 ± 2.5
YGS60 ste4Δrga2Δ1.8 ± 0.5
YGS61 ste4Δbem3Δ1.3 ± 0.1
YGS62 ste4Δrga1Δrga2Δ45.5 ± 2.8
YGS63 ste4Δrga1Δbem3Δ30.3 ± 3.7
YGS64 ste4Δrga2Δbem3Δ3.9 ± 1.2
YGS65 ste4Δrga1Δrga2Δbem3Δ396.1 ± 20.5
  • a All strains are in the SY2002 background.

  • b β-Galactosidase activity was determined as described in Materials and Methods. The reported values are given in 100× Miller units and are averages (± standard deviation) of three determinations.