TABLE 1.

Oligonucleotides

OligonucleotideSequencea
UO5GCGGATCCCTGACCTTACACTGTCGTTGG
UO6GCTCTAGACATTGGCCACTTGTGGAGGTTC
UO9CGGGGTACCCAATACTAGATCCTCTGG
UO10CGGGGATCCAGACTGCAGCCTCTTTTCACAATAGCC
UO11CGGGGATCCGAAGAAGAAGACGATGATG
UO19GCGGATCCATGGCTATTGTGAAAAGAGG
UO20GCAGATCTCATTGGCCACTTGTGGAGGTTC
UO26GCAAGCTTACGCCTGCAGTAGGTAAGAACTTGTTATATTTTG
UO27CTGCAGGCGTAAGCTTGCCCAATCATCATCGTCTTC
UO28GGGATGAGACGAGGTGCAACTTATCAT
UO29GGTGAATGATAAGTTGCACCTCGTCTCATCCC
UO30GGGATGAGACGAGGTGACACTTATCATTCACC
UO31GGTGAATGATAAGTGTCACCTCGTCTCATCCC
UO36CGGGGTACCAAGCTTCCAAAGATTGG
UO37CGGGGATCCAGAGCGGCCGCCCTCTTTTCACAATAGCC
CB20FCGGGCGGCCGCGGTACCTACCTGGAGGTGAGGAG
CB20RCGGGGATCCAATATTTATGAGAAACTATCACTTC
ReverseGGAAACAGCTATGACCATG
  • a Restriction sites used for cloning are underlined in UO5 to UO27 and UO36 to CB20R. Codons mutated for site-directed mutagenesis are underlined in UO28 and UO29.