TABLE 1.

Strains used in this study

StrainRelevant genotypeSource
BY4741 MAT a his3Δ leu2Δ met15Δ ura3ΔRes. Geneticsa
BY4742 MATα his3Δ leu2Δ met15Δ ura3ΔRes. Genetics
BY4743BY4741/BY4742Res. Genetics
VAY27-1C MAT a ade2 trp1 leu2 his3 ura3 can1 F. Cross
VAY27-1A CLN3-PrA::HIS3 (VAY27-1C otherwise)F. Cross
SCMSP75 PGAL-GID8::his3MX/GID8+ (BY4743 otherwise)This study
SCMSP76 PGAL-DCR2::his3MX/DCR2+ (BY4743 otherwise)This study
RPY3 PGAL-DCR2::his3MX (BY4741 otherwise)This study
SCMSP112 gid8Δ::URA3 dcr2Δ::his3MX (BY4741 otherwise)This study
6576 gid8Δ::kanMX (BY4741 otherwise)Res. Genetics
RPY1 dcr2Δ::his3MX (BY4741 otherwise)This study
SCMSP89 DCR2-3HA::kanMX (BY4742 otherwise)This study
SCMSP107 DCR2-13MYC::his3MX (BY4741 otherwise)This study
SCMSP101 GID8-3HA::kanMX (BY4742 otherwise)This study
SCMSP106 GID8-13MYC::his3MX (BY4741 otherwise)This study
SCMSP115 GID8-3HA::kanMX/GID8 DCR2-13MYC::his3MX/DCR2 (BY4743 otherwise)This study
SCMSP116 gid8Δ::URA3 (BY4741 otherwise)This study
SCMSP131 cln3Δ::kanMX gid8Δ::URA3 dcr2Δ::his3MX (BY4741 otherwise)This study
SCMSP123 cln3Δ::kanMX gid8Δ::URA3 (BY4741 otherwise)This study
SCMSP124 swi4Δ::kanMX gid8Δ::URA3 (BY4741 otherwise)This study
SCMSP137 swi4Δ::kanMX gid8Δ::URA3 dcr2Δ::his3MX (BY4741 otherwise)This study
SCMSP134 bck2Δ::kanMX gid8Δ::URA3 (BY4741 otherwise)This study
SCMSP136 bck2Δ::kanMX gid8Δ::URA3 dcr2Δ::his3MX (BY4741 otherwise)This study
SCMSP127 cln3Δ::kanMX dcr2Δ::his3MX (BY4741 otherwise)This study
SCMSP135 bck2Δ::kanMX dcr2Δ::his3MX (BY4741 otherwise)This study
SCMSP128 swi4Δ::kanMX dcr2Δ::his3MX (BY4741 otherwise)This study
YSC1178-7501699 SIC1-TAP::his3MX (BY4741 otherwise)Open Biosystems
10366 cln3Δ::kanMX (BY4742 otherwise)Res. Genetics
16163 bck2Δ::kanMX (BY4742 otherwise)Res. Genetics
16109 swi4Δ::kanMX (BY4742 otherwise)Res. Genetics
36189 bub2Δ::kanMX/bub2Δ::kanMX (BY4743 otherwise)Res. Genetics
31392 mad2Δ::kanMX/mad2Δ::kanMX (BY4743 otherwise)Res. Genetics
36781 mad3Δ::kanMX/mad3Δ::kanMX (BY4743 otherwise)Res. Genetics
  • a Res. Genetics, Research Genetics.