CDR2-lacZ reporter activities mediated by TAC1 in S. cerevisiae and C. albicans

Type of promoter-lacZ fusion (plasmid)Mean ± SD β-galactosidase activity (Miller units)a
Without fluphenazineWith fluphenazine
S. cerevisiae
    CDR2-lacZ2.4 ± 0.4ND
    TAC1-CDR2-lacZ (pDS1157)b96 ± 10ND
    TAC1-CDR2 (lacking DRE)-lacZ (pDS1180)b0.7 ± 0.3ND
    YIp353 (parent plasmid)<0.1ND
C. albicans
    TAC1-CDR2-lacZ (pDS1187)c0.3 ± 0.0514 ± 4
    TAC1-CDR2 (lacking DRE)-lacZ (pDS1190)c<0.1<0.1
    pDS178 (parent plasmid)<0.1<0.1
  • a Values are from three measurements. ND, not determined. Fluphenazine treatment was performed with 20 μg/ml for 60 min.

  • b DSY669 was the recipient of pDS1157 and pDS1180.

  • c DSY2906 was the recipient of pDS1187 and pDS1190.