TABLE 3.

Genetic interactions between GID8 and DCR2

StrainaBudding index (n; P)b
GID8+ DCR2 + (vector-2μ)1 ± 0.12 (19; 1)
GID8 + DCR2 + (GID8-2μ)1.29 ± 0.10 (18; 3 × 10−10)
GID8 + DCR2 + (DCR2-2μ)1.14 ± 0.12 (20; 5 × 10−4)
GID8 + dcr2Δ (GID8-2μ)1.05 ± 0.09 (18; 0.1)
gid8Δ DCR2+ (DCR2-2μ)1.11 ± 0.07 (19; 1 × 10−3)
gid8Δ DCR2+ (vector-2μ)1.03 ± 0.12 (20; 0.5)
GID8 + dcr2Δ (vector-2μ)1.02 ± 0.08 (19; 0.5)
gid8Δ dcr2Δ (vector-2μ)1.01 ± 0.1 (32; 0.8)
GID8 + DCR2 + (vector-2μ)*1 ± 0.05 (30)
GID8 + DCR2 + (GID8-2μ)*1.17 ± 0.05 (30; 7 × 10−11)
GID8 + PGAL-DCR2 (vector-2μ)*1.23 ± 0.10 (30; 8 × 10−9)
GID8 + PGAL-DCR2 (GID8-2μ)*1.22 ± 0.08 (30; 3 × 10−10)
  • a The cells (all in the BY4741 background) were grown in SC media, at 30°C, with glucose or galactose (*) as the carbon source. In these growth conditions, the generation times of all strains were indistinguishable from those of the wild type (94 ± 3 min in glucose-containing media and 165 ± 5 min in galactose-containing media).

  • b The mean and standard deviation of the relative budding index, compared to those for the wild type, are shown in each case. The numbers of individual cultures evaluated (n) and the probabilities associated with Student's t test when the budding indices are compared to that for the wild type are shown in parentheses.