TABLE 4.

Proliferation parameters of CLN3, BCK2, SWI4, GID8, and DCR2 mutantsa

StraingbCell size (μm3)
WTc1 ± 0.0236.6 ± 1.6
gid8Δ1.01 ± 0.0137.1 ± 1.6
dcr2Δ1.01 ± 0.0237.0 ± 1.6
gid8Δ dcr2Δ1 ± 0.0342.0 ± 1.6
bck2Δ1 ± 0.0245.6 ± 1.7
bck2Δ gid8Δ dcr2Δ1 ± 0.0249.5 ± 1.7
cln3Δ1.05 ± 0.0349.2 ± 1.7
cln3Δ gid8Δ dcr2Δ1.05 ± 0.0254.3 ± 1.8
swi4Δ1.08 ± 0.0148.4 ± 1.7
swi4Δ gid8Δ dcr2Δ1.07 ± 0.0156.8 ± 1.9
  • a Cell numbers and cell sizes were obtained with a Coulter counter as described in Materials and Methods. The average and standard deviation from three independent liquid cultures in rich yeast extract-peptone-dextrose media are shown in each case. All the strains were in the haploid BY4741 background.

  • b The generation times (g) of the strains shown are relative to that of wild type, which was 94 ± 1.9 min.

  • c WT, wild type.