TABLE 1.

Strains of S. cerevisiae used in this study

StrainRelevant genotypeReference or source
SGY446MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1::ADE8 tpk2-63(Ts) tpk3::TRP139
AKY54SGY446 (YEp352-BMH2)This study
AKY53SGY446 (YEp352)This study
AKY31SGY446 TPK2 (YEp352)This study
AHY148MATa ura3-52 his3 leu2-3,112 trp1 ade8 tpk1w1 tpk2::HIS3 tpk3::TRP118
RTF1.5-2MATα ura3-52 his3 leu2-3,112 trp1 ade8 tpk1w2 tpk2::HIS3 tpk3::TRP1 bcy1::URA35
AHY240aMATa ura3-52 his3 leu2-3,112 trp1 ade8 TPK1 tpk2::HIS3 tpk3::TRP1 bmh1::ADE8This study
AHT240bMATa ura3-52 his3 leu2-3,112 trp1 ade8 tpk1w1 tpk2::HIS3 tpk3::TRP1 bmh1::ADE8This study
SL1320MATα ura3 leu2 his3 BMH1 bmh2::URA313
SGY459MATα ura3 leu2 his3 pda1::TRP1 bmh1-11(Ts) bmh2::URA3This study
AKY1SGY459(pRS315)This study
AKY2SGY459(pAL110)This study
AKY24SGY459 msn2::HIS3This study
AKY25SGY459 msn2::HIS3 msn4::LEU2This study
AKY5MATα ura3 leu2 his3 bmh1-11(Ts) bmh2::KanMXThis study
AKY9AKY5(pRS315)This study
AKY10AKY5(pAL110)This study
AKY44AKY5 (YEp352)This study
AKY45AKY5 (YEp352-TPK2)This study
AKY46AKY5 (YEp352-TPK3)This study
AKY32AKY5 MSN2::pRS306-MSN2-myc12(pAL110)This study
AKY33AKY5 MSN2::pRS306-MSN2-myc12(pRS315)This study
AKY35SGY559(pWB36)This study
Y3033MATa tap42::HIS3(pRS414-TAP42)9
Y3036Y3033 msn2::LEU2 msn4::URA3This study
Y3034MATa tap42::HIS3 [pRS414-tap42-106]9
Y3037Y3034 msn2::LEU2 msn4::URA3This study
Y3035MATa tap42::HIS3 [pRS414-tap42-106]9
Y3038Y3035 msn2::LEU2 msn4::URA3This study
AKY38Y3033 MSN2::pRS306-MSN2-myc129
AKY79Y3033 pADH-MSN2-GFPThis study
AKY84Y3034 pADH-MSN2-GFPThis study
AKY75AKY38 tip41::KanMXThis study
AKY142AKY79 tip41::KanMXThis study
AKY144AKY84 tip41::KanMXThis study
AKY148MATa tap42::HIS3(pRS414-TAP42)(pAL22)This study
AKY150MATa tap42::HIS3(pRS414-tap42-106)(pAL22)This study
AKY204AKY148 cdc55::NAT1This study
AKY205AKY150 cdc55::NAT1This study
Y2092MATα ade2 his3 leu2 trp1 ura3 GALThis study
Y2762Y2092 pph21::LEU2 pph22::HIS3 GALThis study
AKY101Y2092 MSN2::pRS306-MSN2-myc12This study
AKY103Y2762 MSN2::pRS306-MSN2-myc12This study
BY4742MATα his3Δ leu2Δ lys2Δ ura3ΔResearch Genetics
AKY198BY4742 pADH-MSN2-GFPThis study
AKY163Y2092 rts1::URA3 pADH-MSN2-GFPThis study
AKY185Y2092 cdc55::LEU2 MSN2::pRS306-MSN2-myc12This study
AKY191BY4742 tpd3::KanMX pADH-MSN2-GFPThis study
AKY194AKY79 tpd3::KanMXThis study
AKY195AKY84 tpd3::KanMXThis study