TABLE 1.

Yeast strains

StrainGenotypeSource or reference
JY426 MATa leu2-3,112 ura3-52 his4-34 fus11 fus23Cold Spring Harbor Laboratory
JY429 MATα trp1Δ1 ura3-52 cyh2 fus11 fus23Cold Spring Harbor Laboratory
PJ69-4A MATa trp1-Δ901 leu2-3,112 ura3-52 his3-Δ200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ 25
SEY6210 MATα leu2-3,112 ura3-52 his3200 trp1901 lys2-801 suc29S. Emr
SEY6211 MATa leu2-3,112 ura3-52 his3200 trp1901 ade2 suc29S. Emr
RAY563Same as SEY6210 with sph11::HIS3 2
RAY567Same as SEY6211 with sph11::HIS3 2
RAY914Same as RAY931 with pRS414CDC24 instead of pEG(KT)CDC24 37
RAY916Same as RAY931 with pRS414cdc24-m1 instead of pEG(KT)CDC24 37
RAY918Same as RAY928 with pRS414CDC24 instead of pEG(KT)CDC24 37
RAY928Same as SEY6210 with cdc241::loxP HIS5Spc loxP and pEG(KT)CDC24 37
RAY931Same as SEY6211 with cdc241::loxP HIS5Spc loxP and pEG(KT)CDC24 37
RAY950 MATα leu2-3,112 ura3-52 his3200 trp1901 lys2-801 ade2 cdc24::LEU2 with pRS416GalHis6CDC24 37
RAY1042Same as RAY950 with pRS414CDC24 instead of pRS416GalHis6CDC24 37
RAY1044Same as RAY950 with pRS414cdc24-m1 instead of pRS416GalHis6CDC24 37
RAY1046Same as RAY950 with pRS414cdc24-m2 instead of pRS416GalHis6CDC24 37
RAY1052Same as RAY931 but cdc241::loxP 36
RAY1474Same as RAY1052 with bud1Δ::loxP HIS5SpcloxP 36
RAY1487Same as SEY6211 but cdc24::TRP1 CDC24 and URA3::GFPBUD1 36
RAY1657Same as RAY950 with pRS414cdc24-1 instead of pRS416GalHis6CDC24 34
RAY1660Same as RAY950 with pRS414cdc24-111-1 instead of pRS416GalHis6CDC24 34
RAY1681aSame as RAY950 with pRS414cdc24-m5 instead of pRS416GalHis6CDC24This study
RAY1683aSame as RAY950 with pRS414cdc24-m6 instead of pRS416GalHis6CDC24This study
RAY1685bSame as RAY950 with pRS414cdc24[R416G] instead of pRS416GalHis6CDC24This study
RAY1699Same as RAY950 with pRS414cdc24[E423K/N446I] instead of pRS416GalHis6CDC24This study
RAY1705bSame as RAY950 with pRS414cdc24[E423K] instead of pRS416GalHis6CDC24This study
RAY1716Same as RAY950 with pRS414cdc24[R416G/N551K] instead of pRS416GalHis6CDC24This study
RAY1718Same as RAY950 with pRS414cdc24[R416G/E423K] instead of pRS416GalHis6CDC24This study
RAY1724Same as RAY950 with pRS414cdc24[R416G/E423K/N446I/N551K] instead of pRS416GalHis6CDC24This study
RAY1740bSame as RAY928 with pRS414cdc24[R416G] instead of pEG(KT)CDC24This study
RAY1747bSame as RAY931 with pRS414cdc24[R416G] instead of pEG(KT)CDC24This study
  • a Original isolated mutants.

  • b The cdc24-m6 and cdc24-m5 mutants were recreated by the R416G and E423K mutations, respectively, and were used for all analyses.

  • c HIS5Sp, HIS5 from S. pombe.