TABLE 2.

Frequencies of different septin localization defects in mutant strainsa

StrainRelevant genotype% of cells with defect
Normal neckAbnormal neckMislocalizedAbsent
DLY4790nap1415900
MOSY148cla4167095
DLY4410gin4208000
DLY4687elm197498
DLY3344rga190901
DLY3353rga293601
DLY3346bem393601
DLY4854nap1 cla4177914
DLY4848nap1 gin4377614
DLY4714gin4 elm1184411
DLY4858nap1 elm1781210
DLY4712cla4 elm12542420
DLY4686cla4 gin44541626
DLY4881nap1 rga1256681
DLY4719gin4 rga1379171
DLY4759elm1 rga13631519
DLY3347rga1 rga25528107
DLY3341rga1 bem3741682
DLY3361rga2 bem3811513
DLY4856nap1 cla4 gin40572023
DLY4727cla4 gin4 elm10531631
DLY4855cla4 elm1 nap1064828
DLY4857nap1 gin4 elm109367
DLY4891nap1 rga1 rga258096
DLY4887nap1 rga1 bem35681314
DLY4720gin4 rga1 rga24701412
DLY4931elm1 rga1 rga26541327
DLY4932elm1 rga2 bem33453814
DLY2723rga1 rga2 bem327293211
DLY4859nap1 gin4 elm1 cla40432235
DLY4893nap1 rga1 rga2 bem31491535
DLY4753gin4 rga1 rga2 bem31241758
*belm1 rga1 rga2 bem3
  • a At least 200 cells were scored for each strain.

  • b Segregants of this genotype were identified by accounting for marker segregation in multiple tetrads. Spores germinated and produced a few very large elongated cells but did not form colonies. This genotype was synthetically lethal.