TABLE 1.

Yeast strains used for this studya

StrainRelevant genotypeSource or reference
DLY1a bar128
DLY2723a rga1::TRP1 rga2::URA3 bem3::LEU213
DLY3067a bar1 cdc42::LEU2::GAL1p-CDC4214
DLY3341a rga1::TRP1 bem3::LEU213
DLY3344a rga1::TRP113
DLY3346a bem3::LEU213
DLY3347a rga1::TRP1 rga2::URA313
DLY3353a rga2::URA313
DLY3361a rga2::URA3 bem3::LEU213
DLY4223acdc42V36T,K94E/cdc42::LEU2::GAL1p-CDC4213
DLY4224ahis2::cdc42Y32H::HIS2/his2 cdc42::URA3/cdc42::LEU2::GAL1p-CDC4213
DLY4410α gin49This study
DLY4596a gin49 cdc42::LEU2::GAL1p-CDC42This study
DLY4686a gin49 cla4::TRP1This study
DLY4687a elm1::URA3This study
DLY4712a cla4::TRP1 elm1::URAThis study
DLY4714a gin49 elm1::URA3This study
DLY4719α gin49 rga1::TRP1This study
DLY4720α gin49 rga1::TRP1 rga2::URA3This study
DLY4727a gin49 elm1::URA3 cla4::TRP1This study
DLY4753a gin49 rga1::TRP1 bem3::LEU2 rga2::URA3This study
DLY4759a elm1::URA3 rga1::TRP1This study
DLY4790a bar1nap1::KanrThis study
DLY4848a gin49 nap1::KanrThis study
DLY4854α nap1::Kanr cla4::TRP1This study
DLY4855α nap1::Kanr cla4::TRP1 elm1::URA3This study
DLY4856α nap1::Kanr cla4::TRP1 gin49This study
DLY4857α gin49 nap1::Kanr elm1::URA3This study
DLY4858a nap1::Kanr elm1::URA3This study
DLY4859a nap1::Kanr cla4::TRP1 gin49 elm1::URA3This study
DLY4881α nap1::Kanr rga1::TRP1This study
DLY4887α nap1::Kanr rga1::TRP1 bem3::LEU2This study
DLY4891a nap1::Kanr rga1::TRP1 rga2::URA3This study
DLY4893α nap1::Kanr rga1::TRP1 bem3::LEU2 rga2::URA3This study
DLY4931α elm1::URA3 rga1::TRP1 rga2::URA3This study
DLY4932a elm1::URA3 rga2::URA3 bem3::LEU2This study
MOSY23a cla4::TRP1 cdc42::LEU2::GAL1p-CDC4226
MOSY148a bar1cla4::TRP1This study
  • a All strains are in the BF264-15Du (27) background (ade1 his2 leu2-3,112 trp1-1 ura3Δns).