TABLE 1.

C. neoformans strains created by insertional mutagenesis

StrainPhenotypeaPhenotype linked to nourseothicin resistanceGene(s) flanking insertionMethod of transformationGenBank accession no.
1F2MelbrownYesMPK2, predicted geneBiolisticCG865111
4F2MelleakyNoBiolistic
5D12tsYesWD40, MCM2BiolisticCG865112, CG865113
5E2MelbrownYesPredicted geneBiolisticCG865116
3C12NOhsYesFHB1BiolisticCG865114, CG865115
3E7NOhsYesFHB1Biolistic
7E3MelNoBiolistic
7E12ts, sterileNABiolistic
At-mel1MelYesCLC1Agrobacterium mediatedCG865117, CG865118
At-mel2MelYesLAC1Agrobacterium mediatedCG865119, CG865120
At-mel3MelNoAgrobacterium mediated
  • a ts, reduction in growth at 37°C; Mel, altered melanin production; NOhs, hypersensitivity to nitric oxide.