TABLE 2.

Primers

PrimerSequenceaRestric- tion site
LAE1ATCTACCTTTCTGGGCTCCTGG
LAE2CGTGAAGAACTTGGCGTTGTAG
LA2GACGAGCTCGTGGAACAGTGGAAGGAACSacI
LA3GCGAAGCTTATGAACCGCATCAACCGAHindIII
OEFGCTGTGAAGCTTTGTACCCTGTTTCGCCHindIII
OERGATTTGAAGCTTTGCTGGCATGGAACGGHindIII
MT1ATGCTGAAGCTTGGAAACTGGGAAAGGGGTCHindIII
GFP2TGACGAATTCTCTTAATGGTTTCCTAGCCTGEcoRI
GFP31TGCGGAATTCATGAGCAAGGGCGAGGAAEcoRI
GFP4GGATGCCTCGAGTTTGTACAGCTCGTCCATGCXhoI
GFP5AAGCAGCTCGAGTAAGAGCAAAAGGCGACCACXhoI
GF1CTAGCGAAGCTTGCCACCATGAGCAAGGGCGHindIII
GF2CGGCGAATTCCTTGTACAGCTCGTCCATGCEcoRI
GF3TTTGGAATTCGTTTCGCCGCTGATGTTTGAGEcoRI
FUM1GCGCACTTCTTTGTTTTCCCCT
FUM2CATCGGAATTCTTTCTTGAGCGGCCEcoRI
FUM3TACCAGGATCCAAAACCTCTCGCCABamHI
FUM4CATGACGGTAACTAAGGATTTGG
  • a Underlined sequences show the placement of restriction sites.