TABLE 2.

Results of transformation experiments performed to delete both alleles of BCY1 in C. albicans strain CAI4

Expected genotype (plasmid)aNo. of transformants tested by Southern analysisNo. of transformants of the expected genotype
BCY1bcy1::cat-URA3-cat (pMP1)92
Δbcy1::cat-URA3-catbcy1::cat (pMP1)1130
bcy1::cat-URA3-catbcy1::cat (pMP3)200
BCY1bcy1::cat-URA3-cat Δtpk2::cattpk2::cat (pMP1)165
Δbcy1::cat-URA3-catbcy1::cat Δtpk2::cattpk2::cat (pMP1)319
  • a The transformations were performed with the indicated plasmid.