TABLE 2.

Primers used for UPC2 cassette construction

PrimerSequencea
UPC2-3A5′-AACAGAGCTCTACGTTATTCAGCTTTCC-3′
UPC2-4A5′-TTATGGGCCCACAGTAACGAATCACATTTGTG-3′
UPC2-55′-ATTCCCGCGGCACTGTCATCATCATAAATGGC-3′
UPC2-65′-AGTACTCGAGACTTAATGCAAGGTGATAATGG-3′
UPC2-75′-AGTACTGCAGACTTAATGCAAGGTGATAATGG-3′
  • a Restriction sites introduced into primers are underlined.