TABLE 2.

Sequences of primers used in this study

PrimerSequencea
FRE2−22 5atgtacaattttcaacaCCAAGaagagtttcctaaa
FRE2−22 3atttaggaaactcttCTTGGtgttgaaaattgtaca
FRE2−22 mut5atgtacaattttcaacaGCCGGCagagtttcctaaa
FRE2−22 mut3atttaggaaactctGCCGGCtgttgaaaattgtaca
FRE2−443 5atttttataaattaatGCCAAGccatttttatttct
FRE2−443 3aagaaataaaaatggCTTGGCattaatttataaaaa
FRE2−443 mut5atttttataaattaatGCCGGCccatttttatttct
FRE2−443 mut3aagaaataaaaatggGCCGGCattaatttataaaaa
FRE2−876 5aagaactaattgaaaGCCAAGaaaagttatatggaa
FRE2−876 3attccatataacttttCTTGGCtttcaattagttct
FRE2−876 mut5aagaactaattgaaaGCCGGCaaaagttatatggaa
FRE2−876 mut3attccatataacttttGCCGGCtttcaattagttct
PFRP1−981 5aggtacatcatgaatCCAAGaattaccgatccgatt
PFRP1−981 3aaatcggatcggtaattCTTGGattcatgatgtacc
PFRP1−981 mut 5aggtacatcatgaatGCCGGCattaccgatccgatt
PFRP1−981 mut 3aaatcggatcggtaatGCCGGCttcatgatgtacc
PFRP1−636 5aaacacaacgaagtaGCCAAGaatctggcactagca
PFRP1−636 3atgctagtgccagattCTTGGCtacttcgttgtgtt
PFRP1−636 mut 5aaacacaacgaagtaGCCGGCaatctggcactagca
PFRP1−636 mut 3atgctagtgccagattGCCGGCtacttcgttgtgtt
PFRP1−164 5acgcagttttcggattCTTGGCatagaaacgataaa
PFRP1−164 3aatttatcgtttctatGCCAAGaatccgaaaactgcg
PFRP1−164 mut 5acgcagttttcggattGCCGGCatagaaacgataaa
PFRP1−164 mut 3atttatcgtttctatGCCGGCaatccgaaaactgcg
PFRE2−876 m1ataatagaactaattgaaaGCCGGCaaaagttatatggaattgct
PFRE2−876 m2agcaattccatataacttttGCCGGCtttcaattagttctattat
PFRE2−443 m1tcctttttttataaattaatGCCGGCccatttttatttcttacag
PFRE2−443 m2ctgtaagaaataaaaatggGCCGGCattaatttataaaaaaagga
PFRP1−981 m1cgggaattcgaatGCCGGCattaccgatccgattcctcct
PFRP1−981 m2aggaggaatcggatcggtaatGCCGGCttcatgatgtacc
PFRP1−636 m1aactgaacacaacgaagtaGCCGGCaatctggcactagcaacatc
PFRP1−636 m2gatgttgctagtgccagattGCCGGCtacttcgttgtgttcagtt
PFRP1−164 m1aatttcgcagttttcggattGCCGGCatagaaacgataaatccac
PFRP1−164 m2gtggatttatcgtttctatGCCGGCaatccgaaaactgcgaaatt
FRP1-1ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgatttgatgagcctgtagatgacg
FRP1-2ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattgcacttcctggagcagtaac
FRP1-3ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattaagcaggcatcatccaagta
FRP1-4ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattacattgaccaactaaaccag
FRP1-5ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgatttttgcacagtctggtaataa
FRP1-6ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattcagcagatactaaaagaatc
FRP1-7ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattgttaaacccgaaagaaaagt
FRP1-8ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattgtaaaatttcgcagttttcg
FRP1-9ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgatttgcatgaatgaaaagtatta
LacZClaI+200ctacggaaataatcagccatgtcagc
FRP1-8-1-5a′taaaaatgcttttgtgctgcgtaaaatttc
FRP1-8-1-3a′gaaattttacgcagcacaaaagcattttta
FRP1-8-2-5a′gtaaaatttcgcagttttcggattCTTGGC
FRP1-8-2-3a′GCCAAGaatccgaaaactgcgaaattttac
FRP1-8-3-5a′gattCTTGGCatagaaacgataaatccacc
FRP1-8-3-3a′ggtggatttatcgtttctatGCCAAGaatc
FRP1-8-4-5a′taaatccaccaattaaacgatggccgcatc
FRP1-8-4-3a′gatgcggccatcgtttaattggtggattta
FRP1-8-5-5a′tggccgcatcccttgtctgatgataataag
FRP1-8-5-3a′cttattatcatcagacaagggatgcggcca
FRP1-8-6-5a′tgataataaggtttattgaatgcatgaatg
FRP1-8-6-3a′cattcatgcattcaataaaccttattatca
FRP1-8-4-mut5a′taaatccaTGCGCtaaacgatggccgcatc
FRP1-8-4-mut3a′gatgcggccatcgtttaGCGCAtggattta
HAP5 5DRaacaaacattcaattaagacgaagaaatacagaaagaccccccaaaacaatactcaaacatttcccagtcacgacgtt
HAP5 3DRtattattacaaaatcaaacactatttttaaaatgaacgaaaaaaaaaaaaaaatcctctagtggaattgtgagcggata
HAP5 5detectaaaatgcgacaacggaaagg
HAP5 3detectcaagtgaggaactgagaactg
5′SnaBI CaHIS1gtagtggaagatattctttattgaaaaatagcttgtcaccggatcctggaggatgaggag
3-detecttgtggaattgtgagcggataacaatttcac
HAP41 5DRtacagtatatttattttttcccccatacattaattattctaatcttatgaaattaccatttttcccagtcacgacgtt
HAP41 3DRcatctcatcacatcacatcacatcaaaaataaaatccgttaattttttactgttccttcagtggaattgtgagcggata
HAP43 5DRcaaaaaaaaacaacagagtccaaaaaaatacataataattagaatttcaatttgaacaactttcccagtcacgacgtt
HAP43 3DRgaaaagaaaagaaaaaaaaaactgaagtgtcggaaatacttcatactgtaagtcaaactagtggaattgtgagcggata
HAP41 5detect newccactagttcactcaattac
HAP41 3detect newccaaatcgagcaaaatcaaacc
HAP43 5detecttgtcttccccccaatagact
HAP43 3detecttacctttttgaatacattgt
HAP41UPFWD2NDtgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgactgaaatttctataattttagcagaa
HAP41UPREV2NDttttaaagaaagtagtaccagctcttttttttgtttccgtttataccatccaaatcccgcaatggtaatttcataagattagaat
HAP41DOWNFWDaataattaaataagggtggtaattattactatttacaatcaaaggtggtccttctagatgtgaaggaacagtaaaaaattaacgg
HAP41DOWNREVttacactttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacgtgcgtagtgttcggaagtgttgag
HAP43UPFWDtgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacgctgacacaaacgggaacagaaata
HAP43UPREV2NDttttaaagaaagtagtaccagctcttttttttgtttccgtttataccatccaaatcccgcgttgttcaaattgaaattctaatta
HAP43DOWNFWDaataattaaataagggtggtaattattactatttacaatcaaaggtggtccttctagatgtagtttgacttacagtatgaagtat
HAP43DOWNREVttacactttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcactaaaggtattggtgctgccgcttat
URA3FWD2NDgcgggatttggatggtataaacgg
URA3REVcatctagaaggaccacctttgattg
  • a Uppercase letters denote the endogenous or mutated putative Rim101 binding site.