TABLE 1.

pUG6TG1-3 transformation in different genetic backgrounds of yeast strains

StrainFrequency of G418r eventsa% of circle-mediated telomeric targetingb in G418r events (no. of resistant colonies/total no.)Frequency of circle-mediated telomeric targetingd
Wild type7.90 ± 2.65100 (32/32)7.90 ± 2.65 (0.44)
tlc1 mutant18.6 ± 4.3496.1 (73/76)17.9 ± 4.17 (1.00)
tlc1 rad50 mutant1.10 ± 0.3980.3 (41/51)0.88 ± 0.31 (0.05)
tlc1 rad51 mutant17.0 ± 2.4185.7 (36/42)14.6 ± 2.07 (0.82)
tlc1 type I22.1 ± 2.2100 (39/39)22.1 ± 2.2 (1.23)
tlc1 type II23.8 ± 9.4191.3 (42/46)21.7 ± 8.59 (1.21)
rad52 mutant0.20 ± 0.1285.7 (6/7)0.17 ± 0.10 (0.01)
tlc1 polδ mutant0.00 ± 0.00NAcNA
  • a Results demonstrated as the number of G418-resistant colonies from transformation per microgram of pUG6TG1-3 DNA. Experiments using the control plasmid pRS314 showed that all strains exhibited similar efficiencies for plasmid transformation. The calculation is based on three independent measurements. A standard deviation is given.

  • b The telomeric targeting was determined by Southern analysis using the Kanr gene fragment as a probe, as described for Fig. 2B and C.

  • c Not available (NA) because no G418-resistant colony was recovered from this strain.

  • d The frequency of circle-mediated telomeric targeting was derived by multiplying the values obtained in the previous two columns. Relative frequencies (in parentheses) were calculated by dividing each frequency by that of the tlc1 strain.