TABLE 1.

Effects of different treatment conditions on capsule volume of H99 C. neoformans cellsa

SampleCapsule volume (μm3)
Untreated control1,276 ± 95
DMSO (2 × 15 ml, 30 min each)65 ± 8
Treated with 90 min of radiation in PBS at 14 Gy/min66 ± 3
Suspended in DMSO and irradiated for 90 min at 14 Gy/min (Fig. 1, scheme 1)32 ± 7
Treated with DMSO, extensively washed with PBS, followed by 90 min of radiation in PBS at 14 Gy/min (Fig. 1, scheme 2)33 ± 9
Treated with 90 min of radiation in PBS at 14 Gy/min followed by DMSO treatment (Fig. 1, scheme 3)32 ± 6
  • a Cell volume in all treatment groups was 62 ± 5 μm3. Capsule volume and cell volume are expressed as means with standard errors of the means. Capsule volume was defined as a difference between the volume of the whole cell (yeast cell plus capsule) and the volume of the cell body (as limited by the cell wall).