TABLE 4.

Actin localization in cdc42 mutant cells

StrainCDC42 alleleGrowth conditionsaActin localization (% of cells)b
RandomPolar patchesPolar and cables
AV03E100G30°C6733<1
CaDH50CDC4230°C256510
AV03E100G37°C8217<1
CaDH50CDC4237°C7849
AV03E100G37°C + serum82171
CaDH50CDC4237°C + serum05545
AV06S26I37°C + serum3169<1
AV05S158T37°C + serum47494
  • a A total of 300 rhodamine-phalloidin-stained cells were counted after 4 h of growth at the temperature indicated in minimal medium (+/− 10% FBS).

  • b Random, no polarized actin patches; polar patches, polarized actin patches with no actin cables visible; polar and cables, polarized actin patches and actin cables visible. For details, see text.