TABLE 3.

Hyphal, pseudohyphal, and budded cells in cdc42 mutants

StrainCDC42 alleleGrowth condition% of totala
HyphaePseudohyphaeBudded
Sc5314bCDC4210% FBS9910
CaDH50bCDC4210% FBS86212
AV05S158T10% FBS175132
AV09E100G10% FBS21385
AV06S26I10% FBS33859
Sc5314CDC42Spider601624
CaDH50CDC42Spider462331
AV05S158TSpider01783
AV09E100GSpider0892
AV06S26ISpider0595
  • a Cells were counted for the proportion of buds versus hyphae using DAPI to stain DNA and Calcofluor White to stain the cell wall, in order to ensure that counts reflected the true number of individual cells and that pseudohyphal constrictions would be accentuated by the Calcofluor stain. After 4 h of growth, categories of cells counted were as follows; hyphae were long, filamentous cells with parallel sides and no constrictions visible between cells; pseudohyphae were at least twice the length of a budded cell, with constrictions visible at the cell junctions, or were long filamentous cells with nonparallel sides. In all categories, only nucleated cells (n = 200) were counted.

  • b Sc5314 is a clinical isolate, while CaDH50 is the parental laboratory strain. Both are CDC42/CDC42.