Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About EC
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Eukaryotic Cell
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About EC
    • For Librarians
    • For Advertisers
    • FAQ

Molecular characterization of the putative transcription factor SebA involved in virulence in Aspergillus fumigatus

Taísa Magnani Dinamarco, Ricardo S. Almeida, Patrícia Alves de Castro, Neil Andrew Brown, Thaila Fernanda dos Reis, Leandra Naira Zambelli Ramalho, Marcela Savoldi, Maria Helena S. Goldman, Gustavo Henrique Goldman
Taísa Magnani Dinamarco
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ricardo S. Almeida
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrícia Alves de Castro
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Neil Andrew Brown
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thaila Fernanda dos Reis
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leandra Naira Zambelli Ramalho
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marcela Savoldi
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Maria Helena S. Goldman
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gustavo Henrique Goldman
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: ggoldman@usp.br
DOI: 10.1128/EC.00016-12
  • Article
  • Info & Metrics
  • PDF
Loading

ABSTRACT

Aspergillus fumigatus is a major opportunistic pathogen and allergen of mammals. Nutrient sensing and acquisition mechanisms, as well as the capability to cope with different stressing conditions are essential for A. fumigatus virulence and survival in the mammalian host. This study characterized the A. fumigatus SebA transcription factor, which is the putative homologue of the Trichoderma atroviride seb1. The ΔsebA mutant demonstrated reduced growth in the presence of paraquat, hydrogen peroxide, CaCl2, and poor nutritional conditions, while ΔsebA viability was also affected by heat-shock exposure. Accordingly, SebA::GFP was shown to accumulate in the nucleus upon exposure to oxidative stress and heat-shock conditions. In addition, genes involved in either the oxidative stress or heat-shock response had reduced transcription in the ΔsebA mutant. The A. fumigatus ΔsebA was attenuated in virulence in a murine model of invasive pulmonary aspergillosis. Furthermore, killing of the ΔsebA mutant by murine alveolar macrophages was increased when compared to the wild-type strain. A. fumigatus SebA plays a complex role, contributing to several stress tolerance pathways, growth on poor nutritional conditions and seems to be integrated in different stress responses.

FOOTNOTES

  • Corresponding author: Dr. Gustavo H. Goldman, Laboratório Nacional de Ciência e Tecnologia do Bioetanol – CTBE, and Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão, Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil, Phone/Fax: 55-16-36024280/4281/4311, e-mail address:ggoldman{at}usp.br
  • Copyright © 2012, American Society for Microbiology. All Rights Reserved.
Back to top
Download PDF
Citation Tools
Molecular characterization of the putative transcription factor SebA involved in virulence in Aspergillus fumigatus
Taísa Magnani Dinamarco, Ricardo S. Almeida, Patrícia Alves de Castro, Neil Andrew Brown, Thaila Fernanda dos Reis, Leandra Naira Zambelli Ramalho, Marcela Savoldi, Maria Helena S. Goldman, Gustavo Henrique Goldman
Eukaryotic Cell Feb 2012, EC.00016-12; DOI: 10.1128/EC.00016-12

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Eukaryotic Cell article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Molecular characterization of the putative transcription factor SebA involved in virulence in Aspergillus fumigatus
(Your Name) has forwarded a page to you from Eukaryotic Cell
(Your Name) thought you would be interested in this article in Eukaryotic Cell.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Molecular characterization of the putative transcription factor SebA involved in virulence in Aspergillus fumigatus
Taísa Magnani Dinamarco, Ricardo S. Almeida, Patrícia Alves de Castro, Neil Andrew Brown, Thaila Fernanda dos Reis, Leandra Naira Zambelli Ramalho, Marcela Savoldi, Maria Helena S. Goldman, Gustavo Henrique Goldman
Eukaryotic Cell Feb 2012, EC.00016-12; DOI: 10.1128/EC.00016-12
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About EC
  • For the Media
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1535-9778; Online ISSN: 1535-9786