Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My Cart

Main menu

  • Home
  • Articles
    • Archive
  • About the Journal
    • About EC
    • For Librarians
    • For Advertisers
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Eukaryotic Cell
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Archive
  • About the Journal
    • About EC
    • For Librarians
    • For Advertisers
    • FAQ
Articles

Hos2p/Set3p Deacetylase Complex Signals Secretory Stress through the Mpk1p Cell Integrity Pathway

T. J. Cohen, M. J. Mallory, R. Strich, T.-P. Yao
T. J. Cohen
1Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. J. Mallory
2Department of Molecular Biology, UMDNJ—School of Osteopathic Medicine, Stratford, New Jersey 08084
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. Strich
2Department of Molecular Biology, UMDNJ—School of Osteopathic Medicine, Stratford, New Jersey 08084
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T.-P. Yao
1Department of Pharmacology and Cancer Biology, Duke University, Durham, North Carolina 27710
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: yao00001@mc.duke.edu
DOI: 10.1128/EC.00059-08
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    The Hos2p/Set2p complex is required for the cellular response to tunicamycin-induced stress. (A) Wild-type (wt) and hos2Δ, hda1Δ, rpd3Δ, hos2Δ hda1Δ, and hos2Δ rpd3Δ deletion mutant yeast strains were grown to mid-log phase, adjusted to an optical density of 1, serially diluted 1:10, spotted onto control and 0.2 μg/ml tunicamycin (Tm)-containing plates, and incubated for 48 h. (B) Cells of the hos2Δ mutant strain harboring episomal plasmids containing the vector alone or wild-type HOS2 or an enzymatically inactive hos2Δ (H195,196A) mutant were spotted described as above onto selective medium containing 0.2 μg/ml tunicamycin. (C) Hos2p/Set3p complex deletion strains were evaluated for tunicamycin sensitivity as described above.

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    The Hos2p/Set3p complex is required for a secretory stress response. (A) Hos2p complex deletion strains were spotted as described in the legend to Fig. 1 onto medium containing 2-deoxyglucose or DTT or medium lacking inositol. (B) UPR activation was monitored by Northern analysis from wild-type (wt) or hos2Δ mutant yeast strains challenged with 1 μg/ml tunicamycin (Tm) for 1 h. Northern analysis was performed with a HAC1-specific probe detecting both the full-length (HAC1u) and spliced (HAC1i) isoforms of HAC1. (C) Northern analysis was performed on wild-type and hos2Δ mutant strains treated with tunicamycin for the indicated times with INO1- and KAR2-specific probes. (D) erg6Δ or erg6Δ hos2Δ cells were serially diluted onto complete medium containing 100 μg/ml brefeldin A (BFA), 1 μg/ml bafilomycin A1 (Baf-A1), or 50 μM chlorpromazine (CPZ) for stress sensitivity analysis.

  • FIG. 3.
    • Open in new tab
    • Download powerpoint
    FIG. 3.

    Genetic interactions between hos2Δ and UPR components. Wild-type (wt) and hos2Δ, hac1Δ, and hos2Δ hac1Δ mutant strains were serially diluted, as described in the legend to Fig. 2, onto complete medium at 30 or 37°C or complete medium containing 1 mM hydrogen peroxide (H2O2).

  • FIG. 4.
    • Open in new tab
    • Download powerpoint
    FIG. 4.

    Hos2p is not required for the proper repression of ribosomal gene expression during secretory failure. Northern analysis was performed with the wild-type or hos2Δ mutant strain challenged with 1 μg/ml tunicamycin (Tm) for 0, 1, 2, or 3 h. Northern analysis was performed with labeled probes detecting RPL3, RPL28, and RPL30 previously shown to respond to secretory defects. 28S rRNA served as a loading control.

  • FIG. 5.
    • Open in new tab
    • Download powerpoint
    FIG. 5.

    The Hos2p complex is required for Mpk1p activation following secretory stress. The wild-type (wt) and hos2Δ mutant strains were grown to mid-log phase and challenged with 1 μg/ml tunicamycin (Tm) for 1 h. Western analysis was carried out with antibodies against total Mpk1p and activated phospho-Mpk1p.

  • FIG. 6.
    • Open in new tab
    • Download powerpoint
    FIG. 6.

    Ectopic activation of Mpk1p signaling rescues the hos2Δ secretory growth defect. The wild-type (wt) and hos2Δ and hac1Δ mutant strains harboring the control vector, BCK1-20, or MPK1-2μm plasmid were grown to mid-log phase and serially spotted, as described in the legend to Fig. 5, onto selective medium containing 1 μg/ml tunicamycin (Tm). Plates were incubated at 30°C for 48 h.

  • FIG. 7.
    • Open in new tab
    • Download powerpoint
    FIG. 7.

    Hos2p is not required for secretory stress-induced cyclin C degradation and cell survival but is required for activation of Rlm1p target genes. (A) The wild-type (wt) and hos2Δ mutant strains expressing myc-tagged UME3/SRB11 were grown to mid-log phase, treated with 1 μg/ml tunicamycin (Tm) for 1 h, and harvested for protein analysis by immunoprecipitation-Western blot assay. (B) The wild-type and hos2Δ, cyclinCΔ, and hos2Δ cyclinCΔ mutant strains were serially diluted and spotted onto control and tunicamycin-containing plates. (C) The wild-type and hos2Δ mutant strains were grown to mid-log phase and treated with 1 μg/ml tunicamycin for 4 h. Real-time RT-PCR analysis was performed with primers specific for the Rlm1p target genes YIL117C and MPK1. Values represent mRNA induction levels relative to control actin. Errors are reported as the standard error of the mean.

Tables

  • Figures
  • TABLE 1.

    Yeast strains used in this study

    StrainGenotypeSource or reference
    YDS2 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 44
    AWY1 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hos2::KAN 44
    AWY1202 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hos2::KAN/pAW202 (TRP1) HOS2-13X-Myc 44
    AWY1203 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hos2::KAN/pAW202 (TRP1) HOS2-13X-Myc-H195,196A 44
    JN284 MAT a leu2 his7 ise1 34
    TCY1 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 set3::KANThis study
    TCY2 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 snt1::KANThis study
    TCY3 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sif2::KANThis study
    TCY4 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 cpr1::KANThis study
    TCY5 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 yil112w::KANThis study
    TCY6 MAT a leu2 his7 ise1 hos2::HISThis study
    TCY7 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hos2::KAN hda1::TRPThis study
    TCY8 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hos2::KAN rpd3::TRPThis study
    TCY9 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hac1::TRPThis study
    TCY10 MAT a ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 hos2::KAN hac1::TRPThis study
PreviousNext
Back to top
Download PDF
Citation Tools
Hos2p/Set3p Deacetylase Complex Signals Secretory Stress through the Mpk1p Cell Integrity Pathway
T. J. Cohen, M. J. Mallory, R. Strich, T.-P. Yao
Eukaryotic Cell Jul 2008, 7 (7) 1191-1199; DOI: 10.1128/EC.00059-08

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Email

Thank you for sharing this Eukaryotic Cell article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Hos2p/Set3p Deacetylase Complex Signals Secretory Stress through the Mpk1p Cell Integrity Pathway
(Your Name) has forwarded a page to you from Eukaryotic Cell
(Your Name) thought you would be interested in this article in Eukaryotic Cell.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Hos2p/Set3p Deacetylase Complex Signals Secretory Stress through the Mpk1p Cell Integrity Pathway
T. J. Cohen, M. J. Mallory, R. Strich, T.-P. Yao
Eukaryotic Cell Jul 2008, 7 (7) 1191-1199; DOI: 10.1128/EC.00059-08
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Gene Expression Regulation, Fungal
Histone Deacetylases
Mitogen-Activated Protein Kinases
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Signal Transduction

Related Articles

Cited By...

About

  • About EC
  • For the Media
  • For Librarians
  • For Advertisers
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • Submit a Manuscript to mSphere

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 1535-9778; Online ISSN: 1535-9786