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The Cell Wall of the Human Pathogen Candida glabrata: Differential Incorporation of Novel Adhesin-Like Wall Proteins

Piet W. J. de Groot, Eefje A. Kraneveld, Qing Yuan Yin, Henk L. Dekker, Uwe Groß, Wim Crielaard, Chris G. de Koster, Oliver Bader, Frans M. Klis, Michael Weig
Piet W. J. de Groot
1Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Eefje A. Kraneveld
2Department of Molecular Biology and Preventive Dentistry, Academic Center for Dentistry Amsterdam, Amsterdam, The Netherlands
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Qing Yuan Yin
1Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Henk L. Dekker
1Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Uwe Groß
3Department of Medical Microbiology and National Reference Center for Systemic Mycoses, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany
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Wim Crielaard
2Department of Molecular Biology and Preventive Dentistry, Academic Center for Dentistry Amsterdam, Amsterdam, The Netherlands
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Chris G. de Koster
1Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Oliver Bader
3Department of Medical Microbiology and National Reference Center for Systemic Mycoses, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany
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Frans M. Klis
1Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
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Michael Weig
3Department of Medical Microbiology and National Reference Center for Systemic Mycoses, University Medical Center Göttingen, Kreuzbergring 57, D-37075 Göttingen, Germany
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  • For correspondence: mweig@gwdg.de
DOI: 10.1128/EC.00284-08
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ABSTRACT

The cell wall of the human pathogen Candida glabrata governs initial host-pathogen interactions that underlie the establishment of fungal infections. With the aim of identifying species-specific features that may directly relate to its virulence, we have investigated the cell wall of C. glabrata using a multidisciplinary approach that combines microscopy imaging, biochemical studies, bioinformatics, and tandem mass spectrometry. Electron microscopy revealed a bilayered wall structure in which the outer layer is packed with mannoproteins. Biochemical studies showed that C. glabrata walls incorporate 50% more protein than Saccharomyces cerevisiae walls and, consistent with this, have a higher mannose/glucose ratio. Evidence is presented that C. glabrata walls contain glycosylphosphatidylinositol (GPI) proteins, covalently bound to the wall 1,6-β-glucan, as well as proteins linked through a mild-alkali-sensitive linkage to 1,3-β-glucan. A comprehensive genome-wide in silico inspection showed that in comparison to other fungi, C. glabrata contains an exceptionally large number, 67, of genes encoding adhesin-like GPI proteins. Phylogenetically these adhesin-like proteins form different clusters, one of which is the lectin-like EPA family. Mass spectrometric analysis identified 23 cell wall proteins, including 4 novel adhesin-like proteins, Awp1/2/3/4, and Epa6, which is involved in adherence to human epithelia and biofilm formation. Importantly, the presence of adhesin-like proteins in the wall depended on the growth stage and on the genetic background used, and this was reflected in alterations in adhesion capacity and cell surface hydrophobicity. We propose that the large repertoire of adhesin(-like) genes of C. glabrata contributes to its adaptability and virulence.

  • Copyright © 2008 American Society for Microbiology
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The Cell Wall of the Human Pathogen Candida glabrata: Differential Incorporation of Novel Adhesin-Like Wall Proteins
Piet W. J. de Groot, Eefje A. Kraneveld, Qing Yuan Yin, Henk L. Dekker, Uwe Groß, Wim Crielaard, Chris G. de Koster, Oliver Bader, Frans M. Klis, Michael Weig
Eukaryotic Cell Nov 2008, 7 (11) 1951-1964; DOI: 10.1128/EC.00284-08

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The Cell Wall of the Human Pathogen Candida glabrata: Differential Incorporation of Novel Adhesin-Like Wall Proteins
Piet W. J. de Groot, Eefje A. Kraneveld, Qing Yuan Yin, Henk L. Dekker, Uwe Groß, Wim Crielaard, Chris G. de Koster, Oliver Bader, Frans M. Klis, Michael Weig
Eukaryotic Cell Nov 2008, 7 (11) 1951-1964; DOI: 10.1128/EC.00284-08
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KEYWORDS

Candida glabrata
Candidiasis
Cell Adhesion Molecules
Cell Wall
Fungal Proteins

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