Detection of Fungal DNA in Human Body Fluids and Tissues during a Multistate Outbreak of Fungal Meningitis and Other Infections

DOI: 10.1128/EC.00046-13

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Fig 1
LOD of PCR detection. Serial dilutions of DNA of E. rostratum (A and B), C. cladosporioides (C), and R. laryngis (D) were subjected to DNA purification, PCR, and sequencing. Panels A, C, and D show results of the agarose gel with PCR products obtained using ITS3/4 primers, and panel B shows PCR results obtained using Exserohilum-specific primers. The numbers on the top of the gel depict concentrations of genomic DNA in grams per milliliter.

Table 1

Results of PCR and DNA sequencing presented by patient and by sample

↵a Each case-patient is counted once. Note that some case-patients had more than one specimen type.
↵b FFPE samples are excluded.
↵c These values include 114 case-patients with E. rostratum and 1 case-patient with Cladosporium sp.

Table 2

Comparison of PCR results among serially collected CSF samples (n = 73 case-patients)

Sample type	No. tested
Serial samples have the same results	52
All positive	5
All negative	47
Serial samples have different results	21
First sample positive, then negative	16
First sample negative, then positive	5

Table 3

Comparison between PCR and culture results (n = 139 case-patients)

Results categorized by test performed	No. positive (%)
PCR positive only	27 (19)
Culture positive only	5 (4)
Positive by both PCR and culture	14 (10)
All PCR positive	41 (29)
All culture positive	19 (14)
Positive by either method	46 (33)